ArticleMolecular biology of the cell2004
Regulated exocytosis in neuroendocrine cells: a role for subplasmalemmal Cdc42/N-WASP-induced actin filaments.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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Who cites it
89 citing papers in PubMed.
- Membrane Merger Dynamics in Exocytosis and Endocytosis.Advances in experimental medicine and biology · 2026Review
- A microexon in Arp2 alters tissue-specific Arp2/3-generated actin structures.bioRxiv : the preprint server for biology · 2025Article
- Article
- Assays of Platelet SNARE-actin Interactions.Methods in molecular biology (Clifton, N.J.) · 2025Article
- RhoGDI in RBL-2H3 cells acts as a negative regulator of Rho GTPase signaling to inhibit granule exocytosis.Journal of leukocyte biology · 2024Article
- Trafficking of hormones and trophic factors to secretory and extracellular vesicles: a historical perspective and new hypothesis.Extracellular vesicles and circulating nucleic acids · 2023Article
- Membrane interaction to intercellular spread of pathology in Alzheimer's disease.Frontiers in neuroscience · 2022Review
- Mast cell granule motility and exocytosis is driven by dynamic microtubule formation and kinesin-1 motor function.PloS one · 2022Article
- Review
- Actin and Myosin in Non-Neuronal Exocytosis.Cells · 2020Review
- The receptor tyrosine kinase EPHB6 regulates catecholamine exocytosis in adrenal gland chromaffin cells.The Journal of biological chemistry · 2020Article
- SNARE-Mediated Exocytosis in Neuronal Development.Frontiers in molecular neuroscience · 2020Review
- AMPK phosphorylation of the βAmerican journal of physiology. Renal physiology · 2019Article
- Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis.Journal of cell science · 2018Article
- The fusion pore, 60 years after the first cartoon.FEBS letters · 2018Review
- A Proteomic Variant Approach (ProVarA) for Personalized Medicine of Inherited and Somatic Disease.Journal of molecular biology · 2018Article
- Myosin VI and branched actin filaments mediate membrane constriction and fission of melanosomal tubule carriers.The Journal of cell biology · 2018Article
- Cdc42 controls primary mesenchyme cell morphogenesis in the sea urchin embryo.Developmental biology · 2018Article
- The role of F-actin in the transport and secretion of chromaffin granules: an historic perspective.Pflugers Archiv : European journal of physiology · 2018Review
- Multiple Mechanisms Driving F-actin-Dependent Transport of Organelles to and From Secretory Sites in Bovine Chromaffin Cells.Frontiers in cellular neuroscience · 2018Article
29 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In neuroendocrine cells, actin reorganization is a prerequisite for regulated exocytosis. Small GTPases, Rho proteins, represent potential candidates coupling actin dynamics to membrane trafficking events. We previously reported that Cdc42 plays an active role in regulated exocytosis in chromaffin cells. The aim of the present work was to dissect the molecular effector pathway integrating Cdc42 to the actin architecture required for the secretory reaction in neuroendocrine cells. Using PC12 cells as a secretory model, we show that Cdc42 is activated at the plasma membrane during exocytosis. Expression of the constitutively active Cdc42(L61) mutant increases the secretory response, recruits neural Wiskott-Aldrich syndrome protein (N-WASP), and enhances actin polymerization in the subplasmalemmal region. Moreover, expression of N-WASP stimulates secretion by a mechanism dependent on its ability to induce actin polymerization at the cell periphery. Finally, we observed that actin-related protein-2/3 (Arp2/3) is associated with secretory granules and that it accompanies granules to the docking sites at the plasma membrane upon cell activation. Our results demonstrate for the first time that secretagogue-evoked stimulation induces the sequential ordering of Cdc42, N-WASP, and Arp2/3 at the interface between granules and the plasma membrane, thereby providing an actin structure that makes the exocytotic machinery more efficient.
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