ArticleJournal of bacteriology2003
Identification and characterization of genes required for biosynthesis and transport of the siderophore vibrioferrin in Vibrio parahaemolyticus.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
60 citing papers in PubMed, 118 citations in OpenAlex.
- DFT and TD-DFT studies of free and iron(III)-coordinated vibrioferrin.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2026Article
- Starvation of the bacteriumeLife · 2026Article
- Molecular Adaptations of Ferric Uptake Regulator (Fur) Proteins Drive Iron Homeostasis and Pathogenesis in Vibrio Spp.Current microbiology · 2026Review
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- Phenotypic and Genomic Characterization ofLife (Basel, Switzerland) · 2025Article
- Discovery, functional characterization, and structural studies of the NRPS-independent siderophore synthetases.Critical reviews in biochemistry and molecular biology · 2024Review
- Virulent properties and genomic diversity ofMicrobiology spectrum · 2024Article
- Analysis of the vibrioferrin biosynthetic pathway of Vibrio parahaemolyticus.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2024Article
- Identification ofAntibiotics (Basel, Switzerland) · 2023Article
- The Role of Iron in Phytopathogenic Microbe-Plant Interactions: Insights into Virulence and Host Immune Response.Plants (Basel, Switzerland) · 2023Review
- Genome Sequence ofMicrobiology resource announcements · 2022Article
- A comparative whole-genome approach identifies bacterial traits for marine microbial interactions.Communications biology · 2022Article
- Membrane Efflux Pumps of PathogenicMicroorganisms · 2022Review
- Genome analysis suggests the bacterial family Acetobacteraceae is a source of undiscovered specialized metabolites.Antonie van Leeuwenhoek · 2022Article
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- Secretion Systems in Gram-Negative Bacterial Fish Pathogens.Frontiers in microbiology · 2021Review
- Genetic and structural determinants on iron assimilation pathways in the plant pathogen Xanthomonas citri subsp. citri and Xanthomonas sp.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Review
- FeGenie: A Comprehensive Tool for the Identification of Iron Genes and Iron Gene Neighborhoods in Genome and Metagenome Assemblies.Frontiers in microbiology · 2020Article
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Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In response to low iron availability, Vibrio parahaemolyticus synthesizes and secretes a polyhydroxycarboxylate-type siderophore vibrioferrin which is composed of 1 mol each of 2-ketoglutaric acid, L-alanine, ethanolamine, and citric acid. We have previously reported the cloning and characterization of the pvuA gene, which encodes the 78-kDa outer membrane receptor protein for ferric vibrioferrin. In this study, nine genes involved in the biosynthesis and transport of vibrioferrin have been identified in the genomic regions surrounding the pvuA gene. The genes were sequenced, and gene disruptants were constructed by insertion mutation for phenotype analysis. Five of the genes, named pvsABCDE, constitute an operon that is expressed under iron-limiting conditions. Homology searches of their predicted protein products suggested that the four genes pvsABDE are implicated in the biosynthesis of the siderophore. Another gene in the same operon, pvsC, encodes a putative exporter that is homologous to members of the major facilitator superfamily of multidrug efflux pumps. The remaining four genes, named pvuBCDE, encode proteins strongly homologous to Escherichia coli FecBCDE, respectively, which are components of the ATP-binding cassette transporter system for ferric dicitrate. Reverse transcriptase PCR analysis revealed that these transport genes are transcribed as a single mRNA with the upstream genes, psuA and pvuA. Phenotypic comparison between the wild-type strain and its targeted gene disruptants supported the biological functions for the respective operons that were expected on the basis of the homology search.
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