ArticleJournal of bacteriology2003
Identification and characterization of the nickel uptake system for urease biogenesis in Streptococcus salivarius 57.I.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 56 citations in OpenAlex.
- Urease in acetogenicGut microbes · 2025Article
- Collinsella urealyticum sp. nov., a urease-positive bacterial strain isolated from swine faeces.Archives of microbiology · 2023Article
- Metal Homeostasis in Pathogenic Streptococci.Microorganisms · 2022Review
- Review
- Comparative Genomics ofFrontiers in microbiology · 2019Article
- Distinct Biological Potential of Streptococcus gordonii and Streptococcus sanguinis Revealed by Comparative Genome Analysis.Scientific reports · 2017Article
- The Regulation of the AdcR Regulon in Streptococcus pneumoniae Depends Both on Zn(2+)- and Ni(2+)-Availability.Frontiers in cellular and infection microbiology · 2015Article
- Microbial urease in health and disease.PLoS pathogens · 2014Review
- The pH-dependent expression of the urease operon in Streptococcus salivarius is mediated by CodY.Applied and environmental microbiology · 2014Article
- Phylogenomics and the dynamic genome evolution of the genus Streptococcus.Genome biology and evolution · 2014Article
- Helicobacter hepaticus NikR controls urease and hydrogenase activities via the NikABDE and HH0418 putative nickel import proteins.Microbiology (Reading, England) · 2013Article
- Complete genome sequence of the ureolytic Streptococcus salivarius strain 57.I.Journal of bacteriology · 2011Article
- Geobacter uraniireducens NikR displays a DNA binding mode distinct from other members of the NikR family.Journal of bacteriology · 2010Article
- Comparative genomic analyses of nickel, cobalt and vitamin B12 utilization.BMC genomics · 2009Article
- Interplay of metal ions and urease.Metallomics : integrated biometal science · 2009Review
- Genome of the opportunistic pathogen Streptococcus sanguinis.Journal of bacteriology · 2007Article
- Urea transport in bacteria: acid acclimation by gastric Helicobacter spp.The Journal of membrane biology · 2006Review
- Comparative and functional genomic analysis of prokaryotic nickel and cobalt uptake transporters: evidence for a novel group of ATP-binding cassette transporters.Journal of bacteriology · 2006Article
- Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Ureases are multisubunit enzymes requiring Ni(2+) for activity. The low pH-inducible urease gene cluster in Streptococcus salivarius 57.I is organized as an operon, beginning with ureI, followed by ureABC (structural genes), and ureEFGD (accessory genes). Urease biogenesis also requires a high-affinity Ni(2+) uptake system. By searching the partial genome sequence of a closely related organism, Streptococcus thermophilus LMG18311, three open reading frame (ORFs) homologous to those encoding proteins involved in cobalamin biosynthesis and cobalt transport (cbiMQO) were identified immediately 3' to the ure operon. To determine whether these genes were involved in urease biogenesis by catalyzing Ni(2+) uptake in S. salivarius, regions 3' to ureD were amplified by PCRs from S. salivarius by using primers identical to the S. thermophilus sequences. Sequence analysis of the products revealed three ORFs. Reverse transcriptase PCR was used to demonstrate that the ORFs are transcribed as part of the ure operon. Insertional inactivation of ORF1 with a polar kanamycin marker completely abolished urease activity and the ability to accumulate (63)Ni(2+) during growth. Supplementation of the growth medium with NiCl(2) at concentrations as low as 2.5 micro M partially restored urease activity in the mutant. Both wild-type and mutant strains showed enhanced urease activity when exogenous Ni(2+) was provided at neutral pH. Enhancement of urease activity by adding nickel was regulated at the posttranslational level. Thus, ORF1, ORF2, and ORF3 are part of the ure operon, and these genes, designated ureM, ureQ, and ureO, respectively, likely encode a Ni(2+)-specific ATP-binding cassette transporter.
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