Evidence map›Paper›PMID 14613980›Full record

ArticleGenome research2003

Fugu ESTs: new resources for transcription analysis and genome annotation.

Melody S Clark, Yvonne J K Edwards, Dan Peterson, Sandra W Clifton, Amanda J Thompson, Masahide Sasaki, Yutaka Suzuki, Kiyoshi Kikuchi, Shugo Watabe, Koichi Kawakami and 3 more

Open access · bronzeAbstract readComparative Study
In one paragraph

Article in Genome research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 54 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 1 institution in 1 country.

Melody S ClarkMRC Rosalind Franklin Centre for Genomics Research, (formerly known as the MRC UK HGMP Resource Centre), Genome Campus, Hinxton, Cambridge, CB10 1SB, UK. mscl@bas.ac.uk
Yvonne J K Edwards
Dan Peterson
Sandra W Clifton
Amanda J Thompson
Masahide Sasaki
Yutaka Suzuki
Kiyoshi Kikuchi
Shugo Watabe
Koichi Kawakami
Sumio Sugano
Greg Elgar
Stephen L Johnson
MRC Epidemiology Unit · GB

Funding

INTEGRATED ZEBRAFISH GENOMIC RESOURCESR01DK055379 · NIDDK · WASHINGTON UNIVERSITY · PI JOHNSON, STEPHEN L · 1998 to 2003
$2.9M
NIDDK NIH HHS DK55379
6 · The paper itself

Abstract

The draft Fugu rubripes genome was released in 2002, at which time relatively few cDNAs were available to aid in the annotation of genes. The data presented here describe the sequencing and analysis of 24,398 expressed sequence tags (ESTs) generated from 15 different adult and juvenile Fugu tissues, 74% of which matched protein database entries. Analysis of the EST data compared with the Fugu genome data predicts that approximately 10,116 gene tags have been generated, covering almost one-third of Fugu predicted genes. This represents a remarkable economy of effort. Comparison with the Washington University zebrafish EST assemblies indicates strong conservation within fish species, but significant differences remain. This potentially represents divergence of sequence in the 5' terminal exons and UTRs between these two fish species, although clearly, complete EST data sets are not available for either species. This project provides new Fugu resources, and the analysis adds significant weight to the argument that EST programs remain an essential resource for genome exploitation and annotation. This is particularly timely with the increasing availability of draft genome sequence from different organisms and the mounting emphasis on gene function and regulation.

Indexed as

Expressed Sequence TagsGenomeTranscription, GeneticAnimalsComputational BiologyConserved SequenceDNA, ComplementaryGene LibraryGenesGenetic VariationMultigene FamilyTakifuguZebrafishDNA, Complementary

Identifiers

PMID14613980
PMCPMC403817
OpenAlexW2139344056

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.