ArticleReproductive biology and endocrinology : RB&E2003
Phage integrases for the construction and manipulation of transgenic mammals.
Article in Reproductive biology and endocrinology : RB&E, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 74 citations in OpenAlex.
- Bioengineering Interventions to Enhance the Capacity of the Gut Microbiota in Controlling Food Allergies.Life (Basel, Switzerland) · 2026Review
- A powerful and highly efficient PAI-mediated transgenesis approach in Drosophila.Nucleic acids research · 2025Article
- Development of Humanized Mice in the Age of Genome Editing.Journal of cellular biochemistry · 2017Article
- Multiple expression cassette exchange via TP901-1, R4, and Bxb1 integrase systems on a mouse artificial chromosome.FEBS open bio · 2017Article
- PhiC31/PiggyBac modified stromal stem cells: effect of interferon γ and/or tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) on murine melanoma.Molecular cancer · 2014Article
- Improved site-specific recombinase-based method to produce selectable marker- and vector-backbone-free transgenic cells.Scientific reports · 2014Article
- Hybrid lentivirus-phiC31-int-NLS vector allows site-specific recombination in murine and human cells but induces DNA damage.PloS one · 2014Article
- Article
- Highly parallel assays of tissue-specific enhancers in whole Drosophila embryos.Nature methods · 2013Article
- Cloning, Expression, and in vitro Functional Activity Assay of phiC31 Integrase cDNA in Escherichia coli.Cell journal · 2013Article
- Streptomyces temperate bacteriophage integration systems for stable genetic engineering of actinomycetes (and other organisms).Journal of industrial microbiology & biotechnology · 2012Review
- Engineering the embryo.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Site-specific integrase-mediated transgenesis in mice via pronuclear injection.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector.PloS one · 2011Article
- Article
- Mutational derivatives of PhiC31 integrase with increased efficiency and specificity.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- Bacteriophage phiC31 integrase mediated transgenesis in Xenopus laevis for protein expression at endogenous levels.Methods in molecular biology (Clifton, N.J.) · 2009Article
- Regulated gene insertion by steroid-induced PhiC31 integrase.Nucleic acids research · 2008Article
- Manipulating the Xenopus genome with transposable elements.Genome biology · 2007Review
- Phage Therapy - Everything Old is New Again.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2006Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Phage integrases catalyze site-specific, unidirectional recombination between two short att recognition sites. Recombination results in integration when the att sites are present on two different DNA molecules and deletion or inversion when the att sites are on the same molecule. Here we demonstrate the ability of the phiC31 integrase to integrate DNA into endogenous sequences in the mouse genome following microinjection of donor plasmid and integrase mRNA into mouse single-cell embryos. Transgenic early embryos and a mid-gestation mouse are reported. We also demonstrate the ability of the phiC31, R4, and TP901-1 phage integrases to recombine two introduced att sites on the same chromosome in human cells, resulting in deletion of the intervening material. We compare the frequencies of mammalian chromosomal deletion catalyzed by these three integrases in different chromosomal locations. The results reviewed here introduce these bacteriophage integrases as tools for site-specific modification of the genome for the creation and manipulation of transgenic mammals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.