ArticleThe Biochemical journal2004
A novel Gal(beta1-4)Gal(beta1-4)Fuc(alpha1-6)-core modification attached to the proximal N-acetylglucosamine of keyhole limpet haemocyanin (KLH) N-glycans.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 78 citations in OpenAlex.
- Hemocyanins: Microscopic Giants with Unique Structural Features for Applications in Biomedicine.Vaccines · 2025Review
- Reinvestigation of the internal glycan rearrangement of Lewis a and blood group type H1 epitopes.Physical chemistry chemical physics : PCCP · 2024Article
- Article
- Towards understanding the extensive diversity of protein N-glycan structures in eukaryotes.Biological reviews of the Cambridge Philosophical Society · 2022Review
- Mollusc N-glycosylation: Structures, Functions and Perspectives.Biomolecules · 2021Review
- De Novo Structural Determination of the Oligosaccharide Structure of Hemocyanins from Molluscs.Biomolecules · 2020Review
- Article
- Comparisons of N-glycans across invertebrate phyla.Parasitology · 2019Review
- Ablation of N-acetylglucosaminyltransferases in Caenorhabditis induces expression of unusual intersected and bisected N-glycans.Biochimica et biophysica acta. General subjects · 2018Article
- Identification of multiple isomeric core chitobiose-modified high-mannose and paucimannoseThe Journal of biological chemistry · 2018Article
- Core Richness of N-Glycans of Caenorhabditis elegans: A Case Study on Chemical and Enzymatic Release.Analytical chemistry · 2018Article
- Multistep Fractionation and Mass Spectrometry Reveal Zwitterionic and Anionic Modifications of the N- and O-glycans of a Marine Snail.Molecular & cellular proteomics : MCP · 2016Article
- Positions of the glycans in molluscan hemocyanin, determined by fluorescence spectroscopy.Journal of fluorescence · 2013Article
- Exploring the unique N-glycome of the opportunistic human pathogen Acanthamoeba.The Journal of biological chemistry · 2012Article
- Method for analysis of different oligosacchiride structures.Journal of fluorescence · 2012Article
- Galactosylated fucose epitopes in nematodes: increased expression in a Caenorhabditis mutant associated with altered lectin sensitivity and occurrence in parasitic species.The Journal of biological chemistry · 2012Article
- Complicated N-linked glycans in simple organisms.Biological chemistry · 2012Review
- Glycan structures of the structural subunit (HtH1) of Haliotis tuberculata hemocyanin.Glycoconjugate journal · 2011Article
- Presence of galactosylated core fucose on N-glycans in the planaria Dugesia japonica.Journal of mass spectrometry : JMS · 2011Article
- Evaluation of human antibody responses to keyhole limpet hemocyanin on a carbohydrate microarray.Proteomics. Clinical applications · 2010Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
KLH (keyhole limpet haemocyanin), the oxygen-carrying molecule of the marine snail Megathura crenulata, is often used as an adjuvant or as a hapten carrier for immunizations with peptides, oligosaccharides or other low-molecular-mass organic compounds. KLH exhibits several carbohydrate determinants, at least some of which are immunogenic: it shares an antigenic Fuc(alpha1-3)GalNAc-determinant with schistosomes and contains unique Gal-(beta1-6)Man-structural motifs on its N-glycans. This study reveals the presence of N-glycans with unusual +/-Gal(beta1-4)Gal(beta1-4)Fuc- units (alpha1-6)-linked to the reducing end N -acetylglucosamine residue. The following novel structures of KLH N-glycans were deduced by linkage analysis, exoglycosidase digestion, matrix-assisted laser-desorption ionization-tandem MS and nano-LC-ESI-IT-MS (where LC stands for liquid chromatography, ESI for electrospray ionization and IT for ion trap): Man(alpha1-6)[+/-Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)[Gal(beta1-4)Fuc(alpha1-6)]GlcNAc and Man(alpha1-6)Man(beta1-4)GlcNAc(beta1-4)[Gal(beta1-4)Gal(beta1-4)Fuc(alpha1-6)]GlcNAc. The Gal(beta1-4)Fuc- and Gal(beta1-4)Gal(beta1-4)Fuc- core modifications are expected to be immunogenic, similar to other non-mammalian-type core modifications, and to contribute to the immunostimulatory properties of KLH.
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Registered trials
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