ArticleProceedings of the National Academy of Sciences of the United States of America2003
Dissection of K+ currents in Caenorhabditis elegans muscle cells by genetics and RNA interference.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Shaker/Kv1 potassium channel SHK-1 protects against pathogen infection and oxidative stress in C. elegans.PLoS genetics · 2025Article
- Functional analysis of ctenophore Shaker KBiophysical journal · 2024Article
- Shank promotes action potential repolarization by recruiting BK channels to calcium microdomains.eLife · 2022Article
- Biophysical modeling of C. elegans neurons: Single ion currents and whole-cell dynamics of AWCon and RMD.PloS one · 2019Article
- SLO potassium channels antagonize premature decision making inCommunications biology · 2018Article
- Behavioral Deficits Following Withdrawal from Chronic Ethanol Are Influenced by SLO Channel Function inGenetics · 2017Article
- SLO BK Potassium Channels Couple Gap Junctions to Inhibition of Calcium Signaling in Olfactory Neuron Diversification.PLoS genetics · 2016Article
- KChIP-like auxiliary subunits of Kv4 channels regulate excitability of muscle cells and control male turning behavior during mating in Caenorhabditis elegans.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- SLO-2 potassium channel is an important regulator of neurotransmitter release in Caenorhabditis elegans.Nature communications · 2014Article
- Exciting cell membranes with a blustering heat shock.Biophysical journal · 2014Article
- CLHM-1 is a functionally conserved and conditionally toxic Ca2+-permeable ion channel in Caenorhabditis elegans.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Article
- Action potentials drive body wall muscle contractions in Caenorhabditis elegans.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Genetic dissection of ion currents underlying all-or-none action potentials in C. elegans body-wall muscle cells.The Journal of physiology · 2011Article
- Oxidation of a potassium channel causes progressive sensory function loss during aging.Nature neuroscience · 2009Article
- Mutant analysis of the Shal (Kv4) voltage-gated fast transient K+ channel in Caenorhabditis elegans.The Journal of biological chemistry · 2006Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
GFP-promoter experiments have previously shown that at least nine genes encoding potassium channel subunits are expressed in Caenorhabditis elegans muscle. By using genetic, RNA interference, and physiological techniques we revealed the molecular identity of the major components of the outward K+ currents in body wall muscle cells in culture. We found that under physiological conditions, outward current is dominated by the products of only two genes, Shaker (Kv1) and Shal (Kv4), both expressing voltage-dependent potassium channels. Other channels may be held in reserve to respond to particular circumstances. Because GFP-promoter experiments indicated that slo-2 expression is prominent, we created a deletion mutant to identify the SLO-2 current in vivo. In both whole-cell and single-channel modes, in vivo SLO-2 channels were active only when intracellular Ca2+ and Cl- were raised above normal physiological conditions, as occurs during hypoxia. Under such conditions, SLO-2 is the largest outward current, contributing up to 87% of the total current. Other channels are present in muscle, but our results suggest that they are unlikely to contribute a large outward component under physiological conditions. However, they, too, may contribute currents conditional on other factors. Hence, the picture that emerges is of a complex membrane with a small number of household conductances functioning under normal circumstances, but with additional conductances that are activated during unusual circumstances.
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