ArticleMolecular and cellular biology2003
The DIVa maturase binding site in the yeast group II intron aI2 is essential for intron homing but not for in vivo splicing.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Protein-free catalysis of DNA hydrolysis and self-integration by a ribozyme.Nucleic acids research · 2025Article
- Mobile Bacterial Group II Introns at the Crux of Eukaryotic Evolution.Microbiology spectrum · 2015Review
- Quaternary arrangement of an active, native group II intron ribonucleoprotein complex revealed by small-angle X-ray scattering.Nucleic acids research · 2014Article
- Insights into the strategies used by related group II introns to adapt successfully for the colonisation of a bacterial genome.RNA biology · 2014Review
- Group II intron-ribosome association protects intron RNA from degradation.RNA (New York, N.Y.) · 2013Article
- The brown algae Pl.LSU/2 group II intron-encoded protein has functional reverse transcriptase and maturase activities.PloS one · 2013Article
- Group II introns: mobile ribozymes that invade DNA.Cold Spring Harbor perspectives in biology · 2011Review
- Splicing of the Sinorhizobium meliloti RmInt1 group II intron provides evidence of retroelement behavior.Nucleic acids research · 2011Article
- Genetic identification of potential RNA-binding regions in a group II intron-encoded reverse transcriptase.RNA (New York, N.Y.) · 2010Article
- EcI5, a group IIB intron with high retrohoming frequency: DNA target site recognition and use in gene targeting.RNA (New York, N.Y.) · 2009Article
- The splicing of yeast mitochondrial group I and group II introns requires a DEAD-box protein with RNA chaperone function.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- CRS1, a chloroplast group II intron splicing factor, promotes intron folding through specific interactions with two intron domains.The Plant cell · 2005Article
- High-affinity binding site for a group II intron-encoded reverse transcriptase/maturase within a stem-loop structure in the intron RNA.RNA (New York, N.Y.) · 2004Article
- A horizontally acquired group II intron in the chloroplast psbA gene of a psychrophilic Chlamydomonas: in vitro self-splicing and genetic evidence for maturase activity.RNA (New York, N.Y.) · 2004Article
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6 authors at 2 institutions in 1 country.
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Abstract
Splicing of the Saccharomyces cerevisiae mitochondrial DNA group II intron aI2 depends on the intron-encoded 62-kDa reverse transcriptase-maturase protein (p62). In wild-type strains, p62 remains associated with the excised intron lariat RNA in ribonucleoprotein (RNP) particles that are essential for intron homing. Studies of a bacterial group II intron showed that the DIVa substructure of intron domain IV is a high-affinity binding site for its maturase. Here we first present in vitro evidence extending that conclusion to aI2. Then, experiments with aI2 DIVa mutant strains show that the binding of p62 to DIVa is not essential for aI2 splicing in vivo but is essential for homing. Because aI2 splicing in the DIVa mutant strains remains maturase dependent, splicing must rely on other RNA-protein contacts. The p62 that accumulates in the mutant strains has reverse transcriptase activity, but fractionation experiments at high and low salt concentrations show that it associates more weakly than the wild-type protein with endogenous mitochondrial RNAs, and that phenotype probably explains the homing defect. Replacing the DIVa of aI2 with that of the closely related intron aI1 improves in vivo splicing but not homing, indicating that DIVa contributes to the specificity of the maturase-RNA interaction needed for homing.
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