ArticleMolecular and cellular biology2003
The dynamic mobility of histone H1 is regulated by cyclin/CDK phosphorylation.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 155 citations in OpenAlex.
- A genetic screen for regulators of Drosophila histone H1 binding and chromosome structure in vivo.Genetics · 2026Article
- HMGB1 deforms nucleosomal DNA to generate a dynamic chromatin environment counteracting the effects of linker histone.Science advances · 2025Article
- Calyculin A Induces Premature Chromosome Condensation and Chromatin Compaction in GbioRxiv : the preprint server for biology · 2025Article
- Systematic analysis of the impact of phosphorylation and O-GlcNAcylation on protein subcellular localization.Cell reports · 2023Article
- ATXN3 controls DNA replication and transcription by regulating chromatin structure.Nucleic acids research · 2023Article
- Characterizing crosstalk in epigenetic signaling to understand disease physiology.The Biochemical journal · 2023Article
- Post-Translation Modifications and Mutations of Human Linker Histone Subtypes: Their Manifestation in Disease.International journal of molecular sciences · 2023Review
- Chromatin transitions triggered by LH density as epigenetic regulators of the genome.Nucleic acids research · 2022Article
- Phase separation of DNA: From past to present.Biophysical journal · 2021Review
- Characterization of functional disordered regions within chromatin-associated proteins.iScience · 2021Review
- RanBP2/Nup358 enhances miRNA activity by sumoylating Argonautes.PLoS genetics · 2021Article
- Chromatin and Nuclear Dynamics in the Maintenance of Replication Fork Integrity.Frontiers in genetics · 2021Review
- A novel landscape of nuclear human CDK2 substrates revealed by in situ phosphorylation.Science advances · 2020Article
- Emergence of chromatin hierarchical loops from protein disorder and nucleosome asymmetry.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Priming chromatin for segregation: functional roles of mitotic histone modifications.Cell cycle (Georgetown, Tex.) · 2020Review
- Liquid-Liquid Phase Separation of Histone Proteins in Cells: Role in Chromatin Organization.Biophysical journal · 2020Article
- Highly disordered histone H1-DNA model complexes and their condensates.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Mechanistic role of transglutaminase-2 in focal adhesions.Scientific reports · 2018Article
- Cyclin-dependent kinase 1 (CDK1) and CDK2 have opposing roles in regulating interactions of splicing factor 3B1 with chromatin.The Journal of biological chemistry · 2018Article
- PARP1-dependent eviction of the linker histone H1 mediates immediate early gene expression during neuronal activation.The Journal of cell biology · 2018Article
14 more citing papers are in PubMed but not listed here.
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6 authors at 1 institution in 1 country.
Funding
Abstract
The linker histone H1 is involved in maintaining higher-order chromatin structures and displays dynamic nuclear mobility, which may be regulated by posttranslational modifications. To analyze the effect of H1 tail phosphorylation on the modulation of the histone's nuclear dynamics, we generated a mutant histone H1, referred to as M1-5, in which the five cyclin-dependent kinase phosphorylation consensus sites were mutated from serine or threonine residues into alanines. Cyclin E/CDK2 or cyclin A/CDK2 cannot phosphorylate the mutant in vitro. Using the technique of fluorescence recovery after photobleaching, we observed that the mobility of a green fluorescent protein (GFP)-M1-5 fusion protein is decreased compared to that of a GFP-wild-type H1 fusion protein. In addition, recovery of H1 correlated with CDK2 activity, as GFP-H1 mobility was decreased in cells with low CDK2 activity. Blocking the activity of CDK2 by p21 expression decreased the mobility of GFP-H1 but not that of GFP-M1-5. Finally, the level and rate of recovery of cyan fluorescent protein (CFP)-M1-5 were lower than those of CFP-H1 specifically in heterochromatic regions. These data suggest that CDK2 phosphorylates histone H1 in vivo, resulting in a more open chromatin structure by destabilizing H1-chromatin interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.