ArticleJournal of virology2003
Relationship between RNA lariat debranching and Ty1 element retrotransposition.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- Trichothiodystrophy-associated MPLKIP maintains DBR1 levels for proper lariat debranching and ectodermal differentiation.EMBO molecular medicine · 2023Article
- Saccharomyces cerevisiae RNA lariat debranching enzyme, Dbr1p, is required for completion of reverse transcription by the retrovirus-like element Ty1 and cleaves branched Ty1 RNAs.Molecular genetics and genomics : MGG · 2021Article
- Postsplicing-Derived Full-Length Intron Circles in the Protozoan ParasiteFrontiers in cellular and infection microbiology · 2018Article
- Unexplored Molecular Features of theFrontiers in cellular and infection microbiology · 2018Article
- Conformational Changes in the 5' End of the HIV-1 Genome Dependent on the Debranching Enzyme DBR1 during Early Stages of Infection.Journal of virology · 2017Article
- Arm-specific cleavage and mutation during reverse transcription of 2΄,5΄-branched RNA by Moloney murine leukemia virus reverse transcriptase.Nucleic acids research · 2017Article
- Metal dependence and branched RNA cocrystal structures of the RNA lariat debranching enzyme Dbr1.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- LaSSO, a strategy for genome-wide mapping of intronic lariats and branch points using RNA-seq.Genome research · 2014Article
- Impairment of HIV-1 cDNA synthesis by DBR1 knockdown.Journal of virology · 2014Article
- Structural basis of lariat RNA recognition by the intron debranching enzyme Dbr1.Nucleic acids research · 2014Article
- hDbr1 is a nucleocytoplasmic shuttling protein with a protein phosphatase-like motif essential for debranching activity.Scientific reports · 2013Article
- RNA splicing and debranching viewed through analysis of RNA lariats.Molecular genetics and genomics : MGG · 2011Article
- Isolation and characterization of post-splicing lariat-intron complexes.Nucleic acids research · 2009Article
- The take and give between retrotransposable elements and their hosts.Annual review of genetics · 2008Review
- Ty1 reverse transcriptase does not read through the proposed 2',5'-branched retrotransposition intermediate in vitro.RNA (New York, N.Y.) · 2007Article
- Host factors that control long terminal repeat retrotransposons in Saccharomyces cerevisiae: implications for regulation of mammalian retroviruses.Eukaryotic cell · 2007Review
- DBR1 siRNA inhibition of HIV-1 replication.Retrovirology · 2005Article
- Structure-function analysis of yeast RNA debranching enzyme (Dbr1), a manganese-dependent phosphodiesterase.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Saccharomyces cerevisiae DBR1 gene encodes a 2'-5' phosphodiesterase that debranches intron RNA lariats following splicing. Yeast dbr1 mutants accumulate intron lariats and are also defective for mobility of the retrotransposons Ty1 and Ty3. We used a mutagenic PCR method to generate a collection of dbr1 mutant alleles to explore the relationship between the roles of DBR1 in transposition and debranching. Eight mutants defective for Ty1 transposition contained single amino acid changes in Dbr1p. Two mutations, G84A and N85D, are in a conserved phosphoesterase motif that is believed to be part of the active site of the enzyme, supporting a connection between enzymatic activity and Ty1 transposition. Two other mutations, Y68F and Y68D, occur at a potential phosphorylation site, and we have shown that Dbr1p is phosphorylated on tyrosine. We have developed an RNase protection assay to quantitate intron RNA accumulation in cells. The assay uses RNA probes that hybridize to ACT1 intron RNA. Protection patterns confirm that sequences from the 5' end of the intron to the lariat branch point accumulate in dbr1 mutants in a branched (lariat) conformation. RNase protection assays indicate that all of the newly generated dbr1 mutant alleles are also deficient for debranching, further supporting a role for 2'-5' phosphodiesterase activity in Ty1 transposition. A Ty1 element lacking most of its internal sequences transposes independently of DBR1. The existence of Dbr1p-dependent Ty1 sequences raises the possibility that Dbr1p acts on Ty1 RNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.