ArticleThe EMBO journal2003
Thymine-rich single-stranded DNA activates Mcm4/6/7 helicase on Y-fork and bubble-like substrates.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- Minichromosome maintenance 4 is associated with poor survival and stemness of patients with pancreatic cancer.Medical molecular morphology · 2026Article
- Review
- Multi-omics pan-cancer analyses identify MCM4 as a promising prognostic and diagnostic biomarker.Scientific reports · 2024Article
- Potent DNA strand annealing activity associated with mouse Mcm2∼7 heterohexameric complex.Nucleic acids research · 2017Article
- Mechanisms and regulation of DNA replication initiation in eukaryotes.Critical reviews in biochemistry and molecular biology · 2017Review
- Claspin recruits Cdc7 kinase for initiation of DNA replication in human cells.Nature communications · 2016Article
- DNA induces conformational changes in a recombinant human minichromosome maintenance complex.The Journal of biological chemistry · 2015Article
- Cdc45 (cell division cycle protein 45) guards the gate of the Eukaryote Replisome helicase stabilizing leading strand engagement.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Article
- Biochemical studies of the Saccharomyces cerevisiae Mph1 helicase on junction-containing DNA structures.Nucleic acids research · 2012Article
- Functional cooperation between FACT and MCM is coordinated with cell cycle and differential complex formation.Journal of biomedical science · 2010Article
- Localized melting of duplex DNA by Cdc6/Orc1 at the DNA replication origin in the hyperthermophilic archaeon Pyrococcus furiosus.Extremophiles : life under extreme conditions · 2010Article
- The Mcm complex: unwinding the mechanism of a replicative helicase.Microbiology and molecular biology reviews : MMBR · 2009Review
- Dual DNA unwinding activities of the Rothmund-Thomson syndrome protein, RECQ4.The EMBO journal · 2009Article
- Mcm subunits can assemble into two different active unwinding complexes.The Journal of biological chemistry · 2008Article
- Cdt1 forms a complex with the minichromosome maintenance protein (MCM) and activates its helicase activity.The Journal of biological chemistry · 2008Article
- Specific mutations within the AT-rich region of a plasmid replication origin affect either origin opening or helicase loading.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Binding of AlF-C, an Orc1-binding transcriptional regulator, enhances replicator activity of the rat aldolase B origin.Molecular and cellular biology · 2006Article
- Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells.Molecular biology of the cell · 2006Article
- Functional cooperation between FACT and MCM helicase facilitates initiation of chromatin DNA replication.The EMBO journal · 2006Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The presence of multiple clusters of runs of asymmetric adenine or thymine is a feature commonly found in eukaryotic replication origins. Here we report that the helicase and ATPase activities of the mammalian Mcm4/6/7 complex are activated specifically by thymine stretches. The Mcm helicase is specifically activated by a synthetic bubble structure which mimics an activated replication origin, as well as by a Y-fork structure, provided that a single-stranded DNA region of sufficient length is present in the unwound segment or 3' tail, respectively, and that it carries clusters of thymines. Sequences derived from the human lamin B2 origin can serve as a potent activator for the Mcm helicase, and substitution of its thymine clusters with guanine leads to loss of this activation. At the fork, Mcm displays marked processivity, expected for a replicative helicase. These findings lead us to propose that selective activation by stretches of thymine sequences of a fraction of Mcm helicases loaded onto chromatin may be the determinant for selection of initiation sites on mammalian genomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.