ArticleThe EMBO journal2003
Identification and functional reconstitution of yeast mitochondrial carrier for S-adenosylmethionine.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 119 citations in OpenAlex.
- Unveiling Specificity, Redundancy, and Promiscuity of FiveInternational journal of molecular sciences · 2026Article
- Mechanisms and rationales of SAM homeostasis.Trends in biochemical sciences · 2025Review
- Perturbations in mitochondrial metabolism associated with defective cardiolipin biosynthesis: AnbioRxiv : the preprint server for biology · 2024Article
- Fresh Insights IntoOncology reviews · 2024Review
- Hem25p is required for mitochondrial IPP transport in fungi.Nature cell biology · 2023Article
- Methionine restriction constrains lipoylation and activates mitochondria for nitrogenic synthesis of amino acids.Nature communications · 2023Article
- Whole Genome Sequencing Analysis of Effects of CRISPR/Cas9 inJournal of fungi (Basel, Switzerland) · 2022Article
- Rewiring phospholipid biosynthesis reveals resilience to membrane perturbations and uncovers regulators of lipid homeostasis.The EMBO journal · 2022Article
- Aberrant Expression of Mitochondrial SAM Transporter SLC25A26 Impairs Oocyte Maturation and Early Development in Mice.Oxidative medicine and cellular longevity · 2022Article
- Learning from Yeast about Mitochondrial Carriers.Microorganisms · 2021Review
- Mitochondrial Carriers and Substrates Transport Network: A Lesson fromInternational journal of molecular sciences · 2021Review
- Mitochondrial copper and phosphate transporter specificity was defined early in the evolution of eukaryotes.eLife · 2021Article
- Tricarboxylate Citrate Transporter of an Oleaginous FungusFrontiers in nutrition · 2021Article
- Molecular Mechanism of Citrate Efflux by the Mitochondrial Citrate Transporter CT in Filamentous FungusFrontiers in microbiology · 2021Article
- Harnessing sub-organelle metabolism for biosynthesis of isoprenoids in yeast.Synthetic and systems biotechnology · 2020Review
- Characterization of In Vivo Function(s) of Members of the Plant Mitochondrial Carrier Family.Biomolecules · 2020Review
- Metabolic Roles of Plant Mitochondrial Carriers.Biomolecules · 2020Review
- Diseases Caused by Mutations in Mitochondrial Carrier GenesBiomolecules · 2020Review
- Mechanism-Driven Metabolic Engineering for Bio-Based Production of FreeFrontiers in bioengineering and biotechnology · 2020Article
- Biosynthesis of the nitrogenase active-site cofactor precursor NifB-co inProceedings of the National Academy of Sciences of the United States of America · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genome of Saccharomyces cerevisiae contains 35 members of the mitochondrial carrier protein family, most of which have not yet been functionally identified. Here the identification of the mitochondrial carrier for S-adenosylmethionine (SAM) Sam5p is described. The corresponding gene has been overexpressed in bacteria and the protein has been reconstituted into phospholipid vesicles and identified by its transport properties. In confirmation of its identity, (i) the Sam5p-GFP protein was found to be targeted to mitochondria; (ii) the cells lacking the gene for this carrier showed auxotrophy for biotin (which is synthesized in the mitochondria by the SAM-requiring Bio2p) on fermentable carbon sources and a petite phenotype on non-fermentable substrates; and (iii) both phenotypes of the knock-out mutant were overcome by expressing the cytosolic SAM synthetase (Sam1p) inside the mitochondria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.