ArticleClinical and diagnostic laboratory immunology2003
Measurement of inflammatory biomarkers in synovial tissue extracts by enzyme-linked immunosorbent assay.
Article in Clinical and diagnostic laboratory immunology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 64 citations in OpenAlex.
- Toreforant, an orally active histamine HInflammation research : official journal of the European Histamine Research Society ... [et al.] · 2019Trial
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- General joint hypermobility in temporomandibular joint disease; clinical characteristics, biomarkers, and surgical aspects.Heliyon · 2023Article
- The Triterpenoid Nrf2 Activator, CDDO-Me, Decreases Neutrophil Senescence in a Murine Model of Joint Damage.International journal of molecular sciences · 2023Article
- Cytokines in temporomandibular joint synovial fluid and tissue in relation to inflammation.Journal of oral rehabilitation · 2022Article
- Synovial Tissue Proteins and Patient-Specific Variables as Predictive Factors for Temporomandibular Joint Surgery.Diagnostics (Basel, Switzerland) · 2020Article
- Molecular Surgery: Proteomics of a Rare Genetic Disease Gives Insight into Common Causes of Blindness.iScience · 2020Review
- Penetration of topical diclofenac into synovial tissue and fluid of osteoarthritic knees: a multicenter, randomized, placebo-controlled, pharmacokinetic study.Therapeutic advances in musculoskeletal disease · 2020Article
- Expression Kinetics and Innate Immune Response after Electroporation and LNP-Mediated Delivery of a Self-Amplifying mRNA in the Skin.Molecular therapy. Nucleic acids · 2019Article
- Personalized Proteomics for Precision Health: Identifying Biomarkers of Vitreoretinal Disease.Translational vision science & technology · 2018Article
- Evaluation of antigen-induced synovitis in a porcine model: Immunological, arthroscopic and kinetic studies.BMC veterinary research · 2017Article
- Validation of gene expression biomarker analysis for biopsy-based clinical trials in Crohn's disease.Inflammatory bowel diseases · 2015Article
- Expressions of tumor necrosis factor-α, its receptor I, II and receptor-associated factor 2 in the porcine corpus luteum during the estrous cycle and early pregnancy.Veterinary research communications · 2014Article
- An imprinted rheumatoid arthritis methylome signature reflects pathogenic phenotype.Genome medicine · 2013Article
- Acetate supplementation reduces microglia activation and brain interleukin-1β levels in a rat model of Lyme neuroborreliosis.Journal of neuroinflammation · 2012Article
- Antiinflammatory functions of p38 in mouse models of rheumatoid arthritis: advantages of targeting upstream kinases MKK-3 or MKK-6.Arthritis and rheumatism · 2012Article
- Murine immune responses to a Plasmodium vivax-derived chimeric recombinant protein expressed in Brassica napus.Malaria journal · 2011Article
- The evolving systemic and local biomarker milieu at different stages of disease progression in rat adjuvant-induced arthritis.Journal of clinical immunology · 2009Article
- BDNF in RA: downregulated in plasma following anti-TNF treatment but no correlation with inflammatory parameters.Clinical rheumatology · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed methods for measuring inflammatory biomarkers (cytokines, chemokines, and metalloproteinases) in synovial biopsy specimens from patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Soluble extracts of synovial fragments were prepared with mild detergent and analyzed by enzyme-linked immunosorbent assay (ELISA) for interleukin 1beta (IL-1beta), IL-6, IL-8, tumor necrosis factor alpha (TNF-alpha), and matrix metalloproteinase 3. The optimal detergent was 0.1% Igepal CA-630, which interfered minimally with ELISA detection but extracted 80% of IL-6 from synovial tissue. Upon spiking, 81 to 107% of added biomarkers could be recovered. To determine within-tissue variability, multiple biopsy specimens from each RA synovial extract were analyzed individually. A resulting coefficient of variation of 35 to 62% indicated that six biopsy specimens per synovial extract would result in a sampling error of < or = 25%. Preliminary power analysis suggested that 8 to 15 patients per group would suffice to observe a threefold difference before and after treatment in a serial biopsy clinical study. The previously described significant differences in IL-1beta, IL-6, IL-8, and TNF-alpha levels between RA and OA could be detected, thereby validating the use of synovial extracts for biomarker analysis in arthritis. These methods allow monitoring of biomarker protein levels in synovial tissue and could potentially be applied to early-phase clinical trials to provide a preliminary estimate of drug efficacy.
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