ArticlePlant physiology2003
Methylated DNA-binding proteins from Arabidopsis.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 61 citations in OpenAlex.
- The chromatin reader MBD4 regulates temperature- and flowering-responsive transcription by recognizing histone arginine methylation.Plant communications · 2026Article
- MBD8 is required for LDL2-mediated transcriptional repression downstream of H3K9me2 in Arabidopsis.Nucleic acids research · 2026Article
- Genome-wide identification and expression analysis of the MBD gene family in Broomcorn millet (Panicum miliaceum) and its response to abiotic stress.BMC genomics · 2025Article
- Epigenetic gene regulation in plants and its potential applications in crop improvement.Nature reviews. Molecular cell biology · 2025Review
- Characterization of DNA methylation reader proteins inGenome research · 2024Article
- Genome-Wide Screening and Characterization of Methyl-CpG-Binding Domain (MBD) Proteins inCurrent issues in molecular biology · 2024Article
- MBD2 couples DNA methylation to transposable element silencing during male gametogenesis.Nature plants · 2024Article
- Structural Insights into Methylated DNA Recognition by the Methyl-CpG Binding Domain of MBD6 fromACS omega · 2022Article
- Genome-Wide Characterization of the Methyl CpG Binding Domain-Containing Proteins in Watermelon and Functional Analysis of Their Roles in Disease Resistance Through Ectopic Overexpression inFrontiers in plant science · 2022Article
- MBD5 and MBD6 couple DNA methylation to gene silencing through the J-domain protein SILENZIO.Science (New York, N.Y.) · 2021Article
- Biological role and mechanism of chromatin readers in plants.Current opinion in plant biology · 2021Review
- An Argon-Ion-Induced Pale Green Mutant ofPlants (Basel, Switzerland) · 2021Article
- Overexpression of a methyl-CpG-binding protein gene OsMBD707 leads to larger tiller angles and reduced photoperiod sensitivity in rice.BMC plant biology · 2021Article
- Heterologous Expression ofGenes · 2020Article
- AtMBD4: A methylated DNA binding protein negatively regulates a subset of phosphate starvation genes.Journal of biosciences · 2019Article
- Genome-wide analysis of genes encoding MBD domain-containing proteins from tomato suggest their role in fruit development and abiotic stress responses.Molecular biology reports · 2018Article
- Article
- AtMBD6, a methyl CpG binding domain protein, maintains gene silencing in Arabidopsis by interacting with RNA binding proteins.Journal of biosciences · 2017Article
- The MORC family: new epigenetic regulators of transcription and DNA damage response.Epigenetics · 2013Article
- Deep sequencing on genome-wide scale reveals the unique composition and expression patterns of microRNAs in developing pollen of Oryza sativa.Genome biology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 5-methylcytosines (m5C) play a critical role in epigenetic control, often being recognized by proteins containing a methyl-CpG-binding domain (MBD). Database screening has identified at least 12 putative methyl-CpG-binding proteins from Arabidopsis; we have isolated corresponding cDNAs for seven of them. Despite variation in size and amino acid sequence, all seven proteins exclusively migrate into the nucleus as revealed by green fluorescent protein fusion protein assay, suggesting a relationship with chromatin structure. However, DNA-binding assays using bacterially expressed proteins and synthetic oligonucleotides containing m5C in CpGs showed only one to specifically bind, designated AtMBD5. Further analysis showed that AtMBD5 efficiently binds to m5C in CpNpN (N is A, T, or C) but not in CpNpG sequences, both frequently found in plant DNA. The other six proteins showed either nonspecific DNA binding or no ability to recognize m5C. RNA-blot hybridization and immunoblot analysis indicated AtMBD5 to be present essentially in all tissues. Using green fluorescent protein driven by the authentic promoter, AtMBD5 was found to be actively expressed in pistils and root tips. Because m5Cs in CpG and CpNpN are considered to function in gene expression and gene silencing, respectively, the present results suggest that AtMBD5 may have distinct functions in regulation and/or self defense of genes in actively proliferating cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.