ArticlePlant physiology2003
Blue-light- and phosphorylation-dependent binding of a 14-3-3 protein to phototropins in stomatal guard cells of broad bean.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 150 citations in OpenAlex.
- OmicIntegrator: A Simple and Versatile Tool for Meta-Analysis.Plants (Basel, Switzerland) · 2026Article
- Engineering plant photoreceptors towards enhancing plant productivity.Plant molecular biology · 2025Review
- Expression analysis of genes enriched in the pulvinus ofPlant biotechnology (Tokyo, Japan) · 2025Article
- Phosphorylation of plasma membrane HNature communications · 2024Article
- Light signaling as cellular integrator of multiple environmental cues in plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
- Heterologous expression of flowering locus T promotes flowering but does not affect diurnal movement in the legumePlant biotechnology (Tokyo, Japan) · 2022Article
- Structural and Functional Insights into the Role of Guard Cell Ion Channels in Abiotic Stress-Induced Stomatal Closure.Plants (Basel, Switzerland) · 2021Review
- Regulation of plant phototropic growth by NPH3/RPT2-like substrate phosphorylation and 14-3-3 binding.Nature communications · 2021Article
- Stomatal Responses to Light, COFrontiers in plant science · 2021Article
- Promotion and Upregulation of a Plasma Membrane Proton-ATPase Strategy: Principles and Applications.Frontiers in plant science · 2021Review
- Identification of Abscisic Acid-Dependent Phosphorylated Basic Helix-Loop-Helix Transcription Factors in Guard Cells ofFrontiers in plant science · 2021Article
- Ion Homeostasis and Metabolome Analysis ofFrontiers in plant science · 2021Article
- Protease Inhibitor-Dependent Inhibition of Light-Induced Stomatal Opening.Frontiers in plant science · 2021Article
- Chloroplast avoidance movement: a novel paradigm of ROS signalling.Photosynthesis research · 2020Review
- Role of Proton Motive Force in Photoinduction of Cytoplasmic Streaming inPlants (Basel, Switzerland) · 2020Article
- Blue Light Improves Stomatal Function and Dark-Induced Closure of Rose Leaves (Frontiers in plant science · 2020Article
- Role of Mitochondria in Regulating Lutein and Chlorophyll Biosynthesis inMarine drugs · 2018Article
- Shade-induced nuclear localization of PIF7 is regulated by phosphorylation and 14-3-3 proteins ineLife · 2018Article
- Blue light and CONature communications · 2017Article
- Blue Light Regulation of Stomatal Opening and the Plasma Membrane HPlant physiology · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phototropins are blue-light (BL) receptor serine (Ser)/threonine kinases, and contain two light, oxygen, and voltage (LOV) domains, and are members of the PAS domain superfamily. They mediate phototropism, chloroplast movement, leaf expansion, and stomatal opening of higher plants in response to BL. In stomatal guard cells, genetic analysis has revealed that phototropins mediate activation of the plasma membrane H+-ATPase by phosphorylation and drive stomatal opening. However, biochemical evidence for the involvement of phototropins in the BL response of stomata is lacking. Using guard cell protoplasts, we showed that broad bean (Vicia faba) phototropins (Vfphots) were phosphorylated by BL, and that this phosphorylation of Vfphots reached to the maximum level earlier than that of the H+-ATPase. Phosphorylation of both Vfphots and H+-ATPase showed similar sensitivity to BL and were similarly suppressed by protein kinase and flavoprotein inhibitors. We found that a 14-3-3 protein was bound to Vfphots upon phosphorylation, and this binding occurred earlier than the H+-ATPase phosphorylation. Vfphots (Vfphot1a and Vfphot1b) were expressed in Escherichia coli, and phosphorylation sites were determined to be Ser-358 for Vfphot1a and Ser-344 for Vfphot1b, which are localized between LOV1 and LOV2. We conclude that Vfphots act as BL receptors in guard cells and that phosphorylation of a Ser residue between LOV1 and LOV2 and subsequent 14-3-3 protein binding are likely to be key steps of BL response in stomata. The binding of a 14-3-3 protein to Vfphot was found in etiolated seedlings and leaves in response to BL, suggesting that this event was common to phototropin-mediated responses.
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