ArticlePlant physiology2003
Rubisco activase is required for optimal photosynthesis in the green alga Chlamydomonas reinhardtii in a low-CO(2) atmosphere.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Partitioning of Rubisco activase into the pyrenoidal Rubisco condensate is mediated by a functional protein-protein interaction.Nature communications · 2026Article
- Measurement of Algal Photosynthesis Using a Clark-Type OMethods in molecular biology (Clifton, N.J.) · 2024Article
- Water motion and pH jointly impact the availability of dissolved inorganic carbon to macroalgae.Scientific reports · 2022Article
- Knockout ofJournal of genetics · 2022Article
- Cell adaptation of the extremophilic red microalgaFrontiers in plant science · 2022Article
- Article
- Mixotrophic growth of the extremophile Galdieria sulphuraria reveals the flexibility of its carbon assimilation metabolism.The New phytologist · 2021Article
- Carbon Dioxide Concentration Mechanisms in Natural Populations of Marine Diatoms: Insights FromFrontiers in plant science · 2021Article
- Metabolic, Physiological, and Transcriptomics Analysis of Batch Cultures of the Green MicroalgaCells · 2019Article
- Organelle Studies and Proteome Analyses of Mitochondria and Plastids Fractions from the Diatom Thalassiosira pseudonana.Plant & cell physiology · 2019Article
- Evaluation of strategies for improving the transgene expression in an oleaginous microalga Scenedesmus acutus.BMC biotechnology · 2019Article
- Biosynthetic Conversion of Ag⁺ to highly Stable Ag⁰ Nanoparticles by Wild Type and Cell Wall Deficient Strains ofMolecules (Basel, Switzerland) · 2018Article
- An integrated proteomic and metabolomic study to evaluate the effect of nucleus-cytoplasm interaction in a diploid citrus cybrid between sweet orange and lemon.Plant molecular biology · 2018Article
- Molecular basis of autotrophic vs mixotrophic growth in Chlorella sorokiniana.Scientific reports · 2018Article
- Evolution of Rubisco activase gene in plants.Plant molecular biology · 2018Article
- The Eukaryotic COCell · 2017Article
- A robust protocol for efficient generation, and genomic characterization of insertional mutants ofPlant methods · 2017Article
- Construction of Marker-Free Transgenic Strains of Chlamydomonas reinhardtii Using a Cre/loxP-Mediated Recombinase System.PloS one · 2016Article
- Critical role of Chlamydomonas reinhardtii ferredoxin-5 in maintaining membrane structure and dark metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Alternative acetate production pathways in Chlamydomonas reinhardtii during dark anoxia and the dominant role of chloroplasts in fermentative acetate production.The Plant cell · 2014Article
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5 authors.
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Abstract
This report describes a Chlamydomonas reinhardtii mutant that lacks Rubisco activase (Rca). Using the BleR (bleomycin resistance) gene as a positive selectable marker for nuclear transformation, an insertional mutagenesis screen was performed to select for cells that required a high-CO2 atmosphere for optimal growth. The DNA flanking the BleR insert of one of the high-CO2-requiring strains was cloned using thermal asymmetric interlaced-polymerase chain reaction and inverse polymerase chain reaction and sequenced. The flanking sequence matched the C. reinhardtii Rca cDNA sequence previously deposited in the National Center for Biotechnology Information database. The loss of a functional Rca in the strain was confirmed by the absence of Rca mRNA and protein. The open reading frame for Rca was cloned and expressed in pSL18, a C. reinhardtii expression vector conferring paromomycin resistance. This construct partially complemented the mutant phenotype, supporting the hypothesis that the loss of Rca was the reason the mutant grew poorly in a low-CO2 atmosphere. Sequencing of the C. reinhardtii Rca gene revealed that it contains 10 exons ranging in size from 18 to 470 bp. Low-CO2-grown rca1 cultures had a growth rate and maximum rate of photosynthesis 60% of wild-type cells. Results obtained from experiments on a cia5 rca1 double mutant also suggest that the CO2-concentrating mechanism partially compensates for the absence of an active Rca in the green alga C. reinhardtii.
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