ArticleJournal of clinical microbiology2003
Duplex real-time SYBR green PCR assays for detection of 17 species of food- or waterborne pathogens in stools.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers, 1 of them a synthesis that pooled it.
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Who cites it
52 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Salmonella enterica serovar Typhi and gallbladder cancer: a case-control study and meta-analysis.Cancer medicine · 2016Pooled it
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- Early Warning of Cucumber Angular Leaf Spot by Estimating Airborne Pathogen Aerosols with Particulate Matter Sensors.Plants (Basel, Switzerland) · 2026Article
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- Anti-cancer Properties of Potential Probiotics and Their Cell-free Supernatants for the Prevention of Colorectal Cancer: an In Vitro Study.Probiotics and antimicrobial proteins · 2023Article
- Temporal assessment of N-cycle microbial functions in a tropical agricultural soil using gene co-occurrence networks.PloS one · 2023Article
- Quantitative Assessment of First Nations Drinking Water Distribution Systems for Detection and Prevalence of ThermophilicInternational journal of environmental research and public health · 2022Article
- Detection of Enterotoxigenic Escherichia coli in Rotavirus-Infected Ghanaian Children Diagnosed with Acute Gastroenteritis.The American journal of tropical medicine and hygiene · 2021Article
- Poly(indole-5-carboxylic acid)/reduced graphene oxide/gold nanoparticles/phage-based electrochemical biosensor for highly specific detection of Yersinia pseudotuberculosis.Mikrochimica acta · 2021Article
- SNP Typing Using Multiplex Real-Time PCR Assay for Species Identification of Forensically Important Blowflies and Fleshflies Collected in South Korea (Diptera: Calliphoridae and Sarcophagidae).BioMed research international · 2019Article
- Probiotic supplementation improves reproductive performance of unvaccinated farmed sows infected with porcine epidemic diarrhea virus.Animal science journal = Nihon chikusan Gakkaiho · 2018Article
- Molecular Tools To Study Preharvest Food Safety Challenges.Microbiology spectrum · 2018Review
- Presence and Persistence ofFrontiers in public health · 2018Review
- Intestinal challenge with enterotoxigenicAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2017Review
- Evaluation of PCR Based Assays for the Improvement of Proportion Estimation of Bacterial and Viral Pathogens in Diarrheal Surveillance.Frontiers in microbiology · 2016Article
- Design and Performance Testing of a DNA Extraction Assay for Sensitive and Reliable Quantification of Acetic Acid Bacteria Directly in Red Wine Using Real Time PCR.Frontiers in microbiology · 2016Article
- Novel, In-House, SYBR Green Based One-Step rRT-PCR: Rapid and Accurate Diagnosis of Crimean-Congo Hemorrhagic Fever Virus in Suspected Patients From Iran.Jundishapur journal of microbiology · 2016Article
- Sensitive quantification of Clostridium perfringens in human feces by quantitative real-time PCR targeting alpha-toxin and enterotoxin genes.BMC microbiology · 2015Article
- DNA Sequence Signatures for Rapid Detection of Six Target Bacterial Pathogens Using PCR Assays.Microbiology insights · 2015Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A duplex real-time SYBR Green LightCycler PCR (LC-PCR) assay with DNA extraction using the QIAamp DNA Stool Mini kit was evaluated with regard to detection of 8 of 17 species of food- or waterborne pathogens in five stool specimens in 2 h or less. The protocol used the same LC-PCR with 20 pairs of specific primers. The products formed were identified based on a melting point temperature (T(m)) curve analysis. The 17 species of food- or waterborne pathogens examined were enteroinvasive Escherichia coli, enteropathogenic E. coli, enterohemorrhagic E. coli, enterotoxigenic E. coli, enteroaggregative E. coli, Salmonella spp., Shigella spp., Yersinia enterocolitica, Yersinia pseudotuberculosis, Campylobacter jejuni, Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus, Aeromonas spp., Staphylococcus aureus, Clostridium perfringens, and Bacillus cereus. No interference with the LC-PCR assay was observed when stool specimens were artificially inoculated with each bacterial species. The detection levels were approximately 10(5) food- or waterborne pathogenic bacteria per g of stool. The protocol for processing stool specimens for less than 10(4) food- or waterborne pathogenic bacteria per g of stool requires an overnight enrichment step to achieve adequate sensitivity. However, the rapid amplification and reliable detection of specific genes of greater than 10(5) food- or waterborne pathogenic bacteria per g in samples should facilitate the diagnosis and management of food- or waterborne outbreaks.
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