ArticleJournal of clinical microbiology2003
Detection and differentiation of Mycobacterium tuberculosis and nontuberculous mycobacterial isolates by real-time PCR.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 113 citations in OpenAlex.
- Simultaneous detection and differentiation ofMicrobiology spectrum · 2025Article
- Biochip-Based Identification of Mycobacterial Species in Russia.International journal of molecular sciences · 2024Article
- Description, validation, and review of a decade of experience with a laboratory-developed PCR test for detection ofJournal of clinical tuberculosis and other mycobacterial diseases · 2022Article
- Infectious keratitis: A review.Clinical & experimental ophthalmology · 2022Review
- SARS-CoV-2 Supply Shortages and Tuberculosis Diagnostics: Current Issues Requiring Immediate Solutions.Journal of clinical microbiology · 2021Article
- Diagnosis of anorectal tuberculosis by polymerase chain reaction, GeneXpert and histopathology in 1336 samples in 776 anal fistula patients.World journal of gastrointestinal surgery · 2021Article
- Development of a two-step nucleic acid amplification test for accurate diagnosis of the Mycobacterium tuberculosis complex.Scientific reports · 2021Article
- Article
- Chronic laryngitis caused by Mycobacterium Kansasii in a traveler.The Laryngoscope · 2019Article
- Rapid identification of mycobacteria from positive MGIT broths of primary cultures by MALDI-TOF mass spectrometry.PloS one · 2018Article
- Comparison of histopathology and real-time polymerase chain reaction (RT-PCR) for detection of Mycobacterium tuberculosis in fistula-in-ano.International journal of colorectal disease · 2017Article
- Utility of Real-Time Quantitative Polymerase Chain Reaction in DetectingBioMed research international · 2017Article
- Article
- Validation of a Multiplex Real-Time PCR Assay for Detection of Mycobacterium spp., Mycobacterium tuberculosis Complex, and Mycobacterium avium Complex Directly from Clinical Samples by Use of the BD Max Open System.Journal of clinical microbiology · 2016Article
- Disinfectant-susceptibility of multi-drug-resistant Mycobacterium tuberculosis isolated in Japan.Antimicrobial resistance and infection control · 2016Article
- Evaluation of a low-density hydrogel microarray technique for mycobacterial species identification.Journal of clinical microbiology · 2015Article
- Mycobacterium genotypes in pulmonary tuberculosis infections and their detection by trained African giant pouched rats.Current microbiology · 2015Article
- Rapid and accurate identification of Mycobacterium tuberculosis complex and common non-tuberculous mycobacteria by multiplex real-time PCR targeting different housekeeping genes.Current microbiology · 2012Article
- Design and development of an in-house multiplex RT-PCR assay for simultaneous detection of HIV-1 and HCV in plasma samples.Iranian journal of microbiology · 2012Article
- Multiplex real-time PCR assay and melting curve analysis for identifying Mycobacterium tuberculosis complex and nontuberculous mycobacteria.Journal of clinical microbiology · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycobacteria cause a variety of illnesses that differ in severity and public health implications. The differentiation of Mycobacterium tuberculosis from nontuberculous mycobacteria (NTM) is of primary importance for infection control and choice of antimicrobial therapy. Despite advances in molecular diagnostics, the ability to rapidly diagnose M. tuberculosis infections by PCR is still inadequate, largely because of the possibility of false-negative reactions. We designed and validated a real-time PCR for mycobacteria by using the LightCycler system with 18 reference strains and 168 clinical mycobacterial isolates. All clinically significant mycobacteria were detected; the mean melting temperatures (with 99.9% confidence intervals [99.9% CI] in parentheses) for the different mycobacteria were as follows: M. tuberculosis, 64.35 degrees C (63.27 to 65.42 degrees C); M. kansasii, 59.20 degrees C (58.07 to 60.33 degrees C); M. avium, 57.82 degrees C (57.05 to 58.60 degrees C); M. intracellulare, 54.46 degrees C (53.69 to 55.23 degrees C); M. marinum, 58.91 degrees C (58.28 to 59.55 degrees C); rapidly growing mycobacteria, 53.09 degrees C (50.97 to 55.20 degrees C) or 43.19 degrees C (42.19 to 44.49 degrees C). This real-time PCR assay with melting curve analysis consistently accurately detected and differentiated M. tuberculosis from NTM. Detection of an NTM helps ensure that the negative result for M. tuberculosis is a true negative. The specific melting temperature also provides a suggestion of the identity of the NTM present, when the most commonly encountered mycobacterial species are considered. In a parallel comparison, both the LightCycler assay and the COBAS Amplicor M. tuberculosis assay correctly categorized 48 of 50 specimens that were proven by culture to contain M. tuberculosis, and the LightCycler assay correctly characterized 3 of 3 specimens that contained NTM.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.