ArticleJournal of clinical microbiology2003
Use of O-antigen gene cluster-specific PCRs for the identification and O-genotyping of Yersinia pseudotuberculosis and Yersinia pestis.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 104 citations in OpenAlex.
- Yersinia pseudotuberculosis infection in Kawasaki disease and its clinical characteristics.BMC pediatrics · 2015Trial
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- Draft genome sequence ofMicrobiology resource announcements · 2024Article
- Case report: First isolation ofFrontiers in veterinary science · 2023Article
- Assessment of the Role of Free-Living and Farmed Fallow Deer (Pathogens (Basel, Switzerland) · 2022Article
- Applications of polymerase chain reaction-based methods for the diagnosis of plague (Review).Experimental and therapeutic medicine · 2022Review
- Lipopolysaccharide of theBiomolecules · 2021Review
- Analysis of Yersinia pseudotuberculosis Isolates Recovered from Deceased Mammals of a German Zoo Animal Collection.Journal of clinical microbiology · 2021Article
- Yersinia pseudotuberculosis Prevalence and Diversity in Wild Boars in Northeast Germany.Applied and environmental microbiology · 2018Article
- Phylogeographic separation and formation of sexually discrete lineages in a global population ofMicrobial genomics · 2017Article
- Genetics and evolution of Yersinia pseudotuberculosis O-specific polysaccharides: a novel pattern of O-antigen diversity.FEMS microbiology reviews · 2017Review
- Genetic diversity of the O antigens of Proteus species and the development of a suspension array for molecular serotyping.PloS one · 2017Article
- Mortality in Captive Rhesus Monkeys (Macaca mulatta) in China Due to Infection with Yersinia pseudotuberculosis Serotype O:1a.EcoHealth · 2016Article
- Prevalence of Yersinia enterocolitica and Yersinia pseudotuberculosis in wild boars in the Basque Country, northern Spain.Acta veterinaria Scandinavica · 2016Article
- Vibrio parahaemolyticus Strains of Pandemic Serotypes Identified from Clinical and Environmental Samples from Jiangsu, China.Frontiers in microbiology · 2016Article
- Typing and clustering of Yersinia pseudotuberculosis isolates by restriction fragment length polymorphism analysis using insertion sequences.Journal of clinical microbiology · 2014Article
- Homology analysis of pathogenic Yersinia species Yersinia enterocolitica, Yersinia pseudotuberculosis, and Yersinia pestis based on multilocus sequence typing.Journal of clinical microbiology · 2014Article
- Draft Genome Sequences of Two Yersinia pseudotuberculosis ST43 (O:1b) Strains, B-7194 and B-7195.Genome announcements · 2013Article
- Draft Genome Sequences of Five Yersinia pseudotuberculosis ST19 Isolates and One Isolate Variant.Genome announcements · 2013Article
- Population structure of the Yersinia pseudotuberculosis complex according to multilocus sequence typing.Environmental microbiology · 2011Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Yersinia pestis is a very recently evolved clone of Yersinia pseudotuberculosis serotype O:1b. This close relationship causes potential difficulties in DNA-based diagnostic methods. Analysis of the O-antigen gene clusters in these two organisms identified two regions that were used to specifically identify Y. pestis-Y. pseudotuberculosis as a group or Y. pestis alone. Both PCR assays were found to be 100% specific when tested on a large collection of Yersinia species and other Enterobacteriaceae. Furthermore, advantage was taken of the different setups of the O-antigen gene clusters of the 21 known Y. pseudotuberculosis serotypes to develop a multiplex PCR assay to replace the conventional serotyping method of Y. pseudotuberculosis by O-genotyping. The multiplex PCR assay contained nine sets of specific PCRs in a single tube and when used on Y. pseudotuberculosis reference strains allowed the distinction of 14 individual serotypes and two duplex serotypes (O:4a-O:8 and O:12-O:13). Serotype O:7, O:9, and O:10 strains required additional PCRs for O-genotyping. Once applied to Y. pseudotuberculosis strains of various origins, a very good correlation between classical serotypes and O-genotypes was observed, although some discrepancies were found. O-genotyping also proved useful to correct misidentification of some strains and to type Y. pseudotuberculosis isolates that had lost the expression of the O-antigen. The PCR-based O-genotyping can easily be applied in conventional laboratories, without the need for tedious preparation of a large set of specific antisera.
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