ArticleNucleic acids research2003
High efficiency, site-specific excision of a marker gene by the phage P1 cre-loxP system in the yellow fever mosquito, Aedes aegypti.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Mosquito-based malaria control through genetic, microbial and immunological innovations.Nature reviews. Microbiology · 2026Review
- History of Mosquito Transgenesis: A Perspective in Review.Methods in molecular biology (Clifton, N.J.) · 2025Review
- ImprovedInternational journal of molecular sciences · 2023Article
- Optimizing CRE and PhiC31 mediated recombination inFrontiers in bioengineering and biotechnology · 2023Article
- Making gene drive biodegradable.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Article
- Molecular tools to create new strains for mosquito sexing and vector control.Parasites & vectors · 2018Review
- Highly efficient and inducible DNA excision in transgenic silkworms using the FLP/FRT site-specific recombination system.Transgenic research · 2016Article
- Site-specific cassette exchange systems in the Aedes aegypti mosquito and the Plutella xylostella moth.PloS one · 2015Article
- Article
- Comparison of transgene expression in Aedes aegypti generated by mariner Mos1 transposition and ΦC31 site-directed recombination.Insect molecular biology · 2011Article
- Validation of novel promoter sequences derived from two endogenous ubiquitin genes in transgenic Aedes aegypti.Insect molecular biology · 2010Article
- Homing endonucleases catalyze double-stranded DNA breaks and somatic transgene excision in Aedes aegypti.Insect molecular biology · 2009Article
- Gene expression studies in mosquitoes.Advances in genetics · 2008Review
- High efficiency site-specific genetic engineering of the mosquito genome.Insect molecular biology · 2006Article
- Site-specific genomic targeting in Drosophila.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Utility of the FLP-FRT recombination system for genetic manipulation of rice.Plant cell reports · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
The excision of specific DNA sequences from integrated transgenes in insects permits the dissection in situ of structural elements that may be important in controlling gene expression. Furthermore, manipulation of potential control elements in the context of a single integration site mitigates against insertion site influences of the surrounding genome. The cre-loxP site-specific recombination system has been used successfully to remove a marker gene from transgenic yellow fever mosquitoes, Aedes aegypti. A total of 33.3% of all fertile families resulting from excision protocols showed evidence of cre-loxP-mediated site-specific excision. Excision frequencies were as high as 99.4% within individual families. The cre recombinase was shown to precisely recognize loxP sites in the mosquito genome and catalyze excision. Similar experiments with the FLP/FRT site-specific recombination system failed to demonstrate excision of the marker gene from the mosquito chromosomes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.