ArticleNucleic acids research2003
Quick two-step RNA ligation employing periodate oxidation.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Emerging strategies and innovations in circular RNA synthesis.Molecular therapy. Nucleic acids · 2026Review
- Ultrasensitive miRNA-21 biosensing: a metal-free photoinduced ROMP strategy via 3'-end oxidation.Analytical and bioanalytical chemistry · 2026Article
- cP-RNA-seq for tRNA half sequencing.Methods in enzymology · 2025Article
- Article
- Amine-to-Azide Conversion on Native RNA via Metal-Free Diazotransfer Opens New Avenues for RNA Manipulations.Angewandte Chemie (International ed. in English) · 2021Article
- Amine-to-Azide Conversion on Native RNA via Metal-Free Diazotransfer Opens New Avenues for RNA Manipulations.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2021Article
- G:U-Independent RNA Minihelix Aminoacylation by Nanoarchaeum equitans Alanyl-tRNA Synthetase: An Insight into the Evolution of Aminoacyl-tRNA Synthetases.Journal of molecular evolution · 2020Article
- Selective amplification and sequencing of cyclic phosphate-containing RNAs by the cP-RNA-seq method.Nature protocols · 2016Article
- Mapping interactions between the RNA chaperone FinO and its RNA targets.Nucleic acids research · 2011Article
- Isotope labeling strategies for NMR studies of RNA.Journal of biomolecular NMR · 2010Review
- Multiple segmental and selective isotope labeling of large RNA for NMR structural studies.Nucleic acids research · 2008Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The introduction of modified or labeled nucleotides into RNA is a powerful RNA engineering tool as it enables us to investigate how native RNA modifications affect RNA function and structure. It also helps in the structural analysis of RNA. A modified nucleotide can be introduced into a specific position of RNA by the method of two-step enzymatic ligation of RNA fragments. However, this method requires a complicated purification step between the two ligation steps that results in low yields of the ligation product. Here we have developed a new ligation technique employing periodate oxide that eliminates this purification step. This increases the total yield of the ligation product and makes it a faster procedure.
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Registered trials
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