ArticleNucleic acids research2003
Left-handedly curved DNA regulates accessibility to cis-DNA elements in chromatin.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 35 citations in OpenAlex.
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- Micro-minicircle Gene Therapy: Implications of Size on Fermentation, Complexation, Shearing Resistance, and Expression.Molecular therapy. Nucleic acids · 2014Article
- A designed curved DNA sequence remarkably enhances transgene expression from plasmid DNA in mouse liver.Gene therapy · 2012Article
- Competence of an artificial bent DNA as a transcriptional activator in mouse ES cells.Molecular biology reports · 2011Article
- Activator-to-repressor conversion of T-box transcription factors by the Ripply family of Groucho/TLE-associated mediators.Molecular and cellular biology · 2008Article
- Transient activation of transgene expression by hydrodynamics-based injection may cause rapid decrease in plasmid DNA expression.Gene therapy · 2007Article
- CTCF binds the proximal exonic region of hTERT and inhibits its transcription.Nucleic acids research · 2005Article
- A highly distinctive mechanical property found in the majority of human promoters and its transcriptional relevance.Nucleic acids research · 2005Article
- A determining influence for CpG dinucleotides on nucleosome positioning in vitro.Nucleic acids research · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is little information on chromatin structure that allows access of trans-acting transcription factors. Logically, the target DNA elements become accessible by either exposing themselves towards the environment on the surface of the nucleosome, or making the regulatory region free of the nucleosome. Here, we demonstrate that curved DNA that mimics a negative supercoil can play both roles in the promoter region. By constructing 35 reporter plasmids and using in vivo assay systems, we scrutinized the relationships between upstream DNA geometry, nucleosome positioning and promoter activity. When the left-handedly curved DNA was linked to the herpes simplex virus thymidine kinase (HSV tk) promoter at a specific rotational phase and distance, the curved DNA attracted the nucleosome and the TATA box was thereby left in the linker DNA with its minor groove facing outwards, which led to the activation of transcription. Neither planar curving, nor right-handedly curved DNA nor straight DNA had this effect. Our results seem to provide a clue for solving the problem of why curved DNA is often located near transcriptional control regions.
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Registered trials
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