ArticleNucleic acids research2003
Identification of a new RNA.RNA interaction site for human telomerase RNA (hTR): structural implications for hTR accumulation and a dyskeratosis congenita point mutation.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Distinct Cellular Assembly Stoichiometry of Polycomb Complexes on Chromatin Revealed by Single-molecule Chromatin Immunoprecipitation Imaging.The Journal of biological chemistry · 2015Article
- Predicting structure and stability for RNA complexes with intermolecular loop-loop base-pairing.RNA (New York, N.Y.) · 2014Article
- Structure of active dimeric human telomerase.Nature structural & molecular biology · 2013Article
- Single-molecule analysis of telomerase structure and function.Current opinion in chemical biology · 2011Review
- Ribonucleoprotein multimers and their functions.Critical reviews in biochemistry and molecular biology · 2010Review
- Single-molecule analysis of the human telomerase RNA.dyskerin interaction and the effect of dyskeratosis congenita mutations.Biochemistry · 2009Article
- Two-color fluorescence analysis of individual virions determines the distribution of the copy number of proteins in herpes simplex virus particles.Biophysical journal · 2007Article
- Telomere restoration and extension of proliferative lifespan in dyskeratosis congenita fibroblasts.Aging cell · 2007Article
- Structural analysis of the catalytic core of human telomerase RNA by FRET and molecular modeling.Biochemistry · 2006Article
- Analysis of human telomerase activity and function by two color single molecule coincidence fluorescence spectroscopy.Journal of the American Chemical Society · 2006Article
- Dyskeratosis congenita: a disorder of defective telomere maintenance?International journal of hematology · 2005Review
- Combined effects of Cantide and chemotherapeutic drugs on inhibition of tumor cells' growth in vitro and in vivo.World journal of gastroenterology · 2005Article
- A human-Tetrahymena pseudoknot chimeric telomerase RNA reconstitutes a nonprocessive enzyme in vitro that is defective in telomere elongation.Nucleic acids research · 2005Article
- Measuring single-molecule nucleic acid dynamics in solution by two-color filtered ratiometric fluorescence correlation spectroscopy.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Fluorescence-aided molecule sorting: analysis of structure and interactions by alternating-laser excitation of single molecules.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Telomerase dysfunction and dyskeratosis congenita.Cytotechnology · 2004Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The enzyme telomerase is a ribonucleoprotein that has a critical role in the maintenance of stable telomeres in organisms that possess linear chromosomes. Using a recently developed single molecule fluorescence coincidence method, we have studied the RNA component of telomerase (hTR) and directly observed multimerisation of hTR in solution. RNA mutagenesis and blocking oligonucleotides were employed to identify the single-stranded internal loop J7b/8a as an important and specific hTR.hTR interaction site. This observation was confirmed by studies on a model RNA fragment (hTR(380-444)), comprising part of the H/ACA domain, the internal loop J7b/8a and the CR7 domain, that was found to dimerise. Substitution mutagenesis within the proposed RNA.RNA interaction site of hTR(380-444) resulted in a loss of dimerisation potential and insertion of the dyskeratosis congenita mutation C408G led to a significant reduction in dimer formation. Together, these results suggest that this RNA.RNA interaction site may be functionally relevant.
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