ArticleNucleic acids research2003
Arginine/serine repeats are sufficient to constitute a splicing activation domain.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- Transcript isoform switching during embryonic and post-hatch development of broiler breast muscle.Poultry science · 2026Article
- Persulfidation of the zinc finger protein ZRANB2 modulates its RNA binding and alternative splicing function.Journal of inorganic biochemistry · 2026Article
- RNA splicing: Novel star in pulmonary diseases with a treatment perspective.Acta pharmaceutica Sinica. B · 2025Review
- Zinc and RNA Binding Is Linked to the Conformational Flexibility of ZRANB2: A CCCC-Type Zinc Finger Protein.Biochemistry · 2025Article
- An autoantibody signature predictive for multiple sclerosis.Nature medicine · 2024Article
- Function of BCLAF1 in human disease.Oncology letters · 2022Review
- Non-invasive and high-throughput interrogation of exon-specific isoform expression.Nature cell biology · 2021Article
- Identification and Functional Analysis of the Sex-DeterminerFrontiers in physiology · 2021Article
- Versatility of Cyclophilins in Plant Growth and Survival: A Case Study inBiomolecules · 2019Review
- Modeling and Predicting the Activities of Trans-Acting Splicing Factors with Machine Learning.Cell systems · 2018Article
- C-terminal short arginine/serine repeat sequence-dependent regulation of Y14 (RBM8A) localization.Scientific reports · 2018Article
- Expansion of Lysine-rich Repeats in Plasmodium Proteins Generates Novel Localization Sequences That Target the Periphery of the Host Erythrocyte.The Journal of biological chemistry · 2016Article
- LUC7L3/CROP inhibits replication of hepatitis B virus via suppressing enhancer II/basal core promoter activity.Scientific reports · 2016Article
- The adipogenic transcriptional cofactor ZNF638 interacts with splicing regulators and influences alternative splicing.Journal of lipid research · 2014Article
- SRSF1 and SRSF9 RNA binding proteins promote Wnt signalling-mediated tumorigenesis by enhancing β-catenin biosynthesis.EMBO molecular medicine · 2013Article
- Article
- Engineering splicing factors with designed specificities.Nature methods · 2009Article
- Deletion of the N-terminus of SF2/ASF permits RS-domain-independent pre-mRNA splicing.PloS one · 2007Article
- The impact of alternative splicing in vivo: mouse models show the way.RNA (New York, N.Y.) · 2007Review
- Multiple aromatic side chains within a disordered structure are critical for transcription and transforming activity of EWS family oncoproteins.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
SR proteins are essential pre-mRNA splicing factors that have been shown to bind a number of exonic splicing enhancers where they function to stimulate the splicing of adjacent introns. Members of the SR protein family contain one or two N-terminal RNA binding domains, as well as a C-terminal arginine-serine (RS) rich domain. The RS domains mediate protein-protein interactions with other RS domain containing proteins and are essential for many, but not all, SR protein functions. Hybrid proteins containing an RS domain fused to the bacteriophage MS2 coat protein are sufficient to activate enhancer-dependent splicing in HeLa cell nuclear extract when bound to the pre-mRNA. Here we report progress towards determining the protein sequence requirements for RS domain function. We show that the RS domains from non-SR proteins can also function as splicing activation domains when tethered to the pre-mRNA. Truncation experiments with the RS domain of the human SR protein 9G8 identified a 29 amino acid segment, containing 26 arginine or serine residues, that is sufficient to activate splicing when fused to MS2. We also show that synthetic domains composed solely of RS dipeptides are capable of activating splicing, although their potency is proportional to their size.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.