ArticleApplied and environmental microbiology2003
Analysis, characterization, and loci of the tuf genes in lactobacillus and bifidobacterium species and their direct application for species identification.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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57 citing papers in PubMed, 158 citations in OpenAlex.
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- Mapping the Key Technological and Functional Characteristics of Indigenous Lactic Acid Bacteria Isolated from Greek Traditional Dairy Products.Microorganisms · 2022Article
- A Predictive Growth Model for Pro-technological and ProbioticFrontiers in microbiology · 2022Article
- Probiotic Lactobacilli in Fermented Dairy Products: Selective Detection, Enumeration and Identification Scheme.Microorganisms · 2021Article
- Metagenetic Analysis for Microbial Characterization ofFoods (Basel, Switzerland) · 2021Article
- Antimicrobial properties of Lactobacillus cell-free supernatants against multidrug-resistant urogenital pathogens.MicrobiologyOpen · 2021Article
- Isolation of aBiomedicines · 2020Article
- Phylogenetic analysis of Leuconostoc and Lactobacillus species isolated from sugarcane processing streams.MicrobiologyOpen · 2020Article
- Diagnosis of human and canineHeliyon · 2020Article
- Revisiting the Metabolic Capabilities ofMicroorganisms · 2020Article
- Molecular Routes to Specific Identification of the Lactobacillus Casei Group at the Species, Subspecies and Strain Level.International journal of molecular sciences · 2020Article
- Glutamine synthetase type I (glnAI) represents a rewarding molecular marker in the classification of bifidobacteria and related genera.Folia microbiologica · 2020Article
- Lactic Acid Bacteria Isolation from Spontaneous Sourdough and Their Characterization Including Antimicrobial and Antifungal Properties Evaluation.Microorganisms · 2019Article
- Use of a SelectedFoods (Basel, Switzerland) · 2019Article
- Effect of Sequential Inoculum of Beta-Glucosidase Positive and Probiotic Strains on Brine Fermentation to Obtain Low Salt Sicilian Table Olives.Frontiers in microbiology · 2019Article
- Application of A Novel Potential ProbioticMicroorganisms · 2018Article
- The threonine-tRNA ligase gene region is applicable in classification, typing, and phylogenetic analysis of bifidobacteria.Journal of microbiology (Seoul, Korea) · 2018Article
- A Transposon Mutagenesis System for Bifidobacterium longum subsp. longum Based on an ISApplied and environmental microbiology · 2018Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We analyzed the tuf gene, encoding elongation factor Tu, from 33 strains representing 17 Lactobacillus species and 8 Bifidobacterium species. The tuf sequences were aligned and used to infer phylogenesis among species of lactobacilli and bifidobacteria. We demonstrated that the synonymous substitution affecting this gene renders elongation factor Tu a reliable molecular clock for investigating evolutionary distances of lactobacilli and bifidobacteria. In fact, the phylogeny generated by these tuf sequences is consistent with that derived from 16S rRNA analysis. The investigation of a multiple alignment of tuf sequences revealed regions conserved among strains belonging to the same species but distinct from those of other species. PCR primers complementary to these regions allowed species-specific identification of closely related species, such as Lactobacillus casei group members. These tuf gene-based assays developed in this study provide an alternative to present methods for the identification for lactic acid bacterial species. Since a variable number of tuf genes have been described for bacteria, the presence of multiple genes was examined. Southern analysis revealed one tuf gene in the genomes of lactobacilli and bifidobacteria, but the tuf gene was arranged differently in the genomes of these two taxa. Our results revealed that the tuf gene in bifidobacteria is flanked by the same gene constellation as the str operon, as originally reported for Escherichia coli. In contrast, bioinformatic and transcriptional analyses of the DNA region flanking the tuf gene in four Lactobacillus species indicated the same four-gene unit and suggested a novel tuf operon specific for the genus Lactobacillus.
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