ArticleHistochemistry1992
Comparison of 35S- and digoxigenin-labeled RNA and oligonucleotide probes for in situ hybridization. Expression of mRNA of the seminal vesicle secretion protein II and androgen receptor genes in the rat prostate.
Article in Histochemistry, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 128 citations in OpenAlex.
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- Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization.Journal of visualized experiments : JoVE · 2017Article
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- LINE-1 family member GCRG123 gene is up-regulated in human gastric signet-ring cell carcinoma.World journal of gastroenterology · 2008Article
- EphB3 receptor and ligand expression in the adult rat brain.Journal of molecular histology · 2006Article
- A novel RNA-binding protein in neuronal RNA granules: regulatory machinery for local translation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- A gene encoding an apurinic/apyrimidinic endonuclease-like protein is up-regulated in human gastric cancer.World journal of gastroenterology · 2003Article
- Expression of the FAT10 gene is highly upregulated in hepatocellular carcinoma and other gastrointestinal and gynecological cancers.Oncogene · 2003Article
- A novel gene, GCRG224, is differentially expressed in human gastric mucosa.World journal of gastroenterology · 2003Article
- Interleukin-1beta expression in human gastric carcinoma with Epstein-Barr virus infection.Journal of virology · 2002Article
- Cloning and characterization of PIMT, a protein with a methyltransferase domain, which interacts with and enhances nuclear receptor coactivator PRIP function.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Impaired endochondral ossification and angiogenesis in mice deficient in membrane-type matrix metalloproteinase I.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Kinesin-like protein CENP-E is upregulated in rheumatoid synovial fibroblasts.Arthritis research · 1999Article
- Lack of association between enhanced TRPM-2/clusterin expression and increased apoptotic activity in sex-hormone-induced prostatic dysplasia of the Noble rat.The American journal of pathology · 1998Article
- In situ hybridization studies of metalloproteinases 2 and 9 and TIMP-1 and TIMP-2 expression in human prostate cancer.Clinical & experimental metastasis · 1997Article
- In situ hybridization: methods and applications.Journal of clinical laboratory analysis · 1997Review
- Kinetics of Fas-induced apoptosis in thymic organ culture.Journal of clinical immunology · 1997Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
The sensitivity of radiolabeled and digoxigenin-labeled RNA probes and synthetic oligonucleotide probes for the detection of seminal vesicle secretion protein II (SVS II) and androgen receptor (AR) mRNA was compared by in situ hybridization in paraformaldehyde-fixed cryostat sections of the rat prostate. Both genes are expressed in different amounts in the various prostatic lobes and contiguous glands. SVS II or AR RNA probes were either labeled with digoxigenin-11-UTP or [35S]UTP by in vitro transcription. A synthetic SVS II oligonucleotide probe was 3' end-labeled (tailed) with either digoxigenin-11-dUTP or [35S]dATP. Hybridized 35S-labeled probes were detected by autoradiography and digoxigenin-labeled probes by immunohistochemistry using alkaline phosphatase conjugated anti-digoxigenin antibody or gold-labeled antibody followed by protein A-gold and silver enhancement. Digoxigenin-labeled probes provided the same degree of sensitivity as their 35S-labeled counterparts for the detection by in situ hybridization of weakly and strongly expressed mRNA. Using both labeling methods, the SVS II RNA probes were more sensitive than the oligonucleotide probes and background labelling of the 35S-labeled oligonucleotide probe was high. The digoxigenin method produced less background with all probe types, hybridization signals showed higher resolution and results were obtained faster than with radiolabeled probes. The immunogold silver enhancement system provided the fastest detection of digoxigenin-labeled probes with a sensitivity and resolution similar to that provided by alkaline phosphatase anti-digoxigenin immunohistochemistry. It is concluded that digoxigenin probe labeling and detection provides a sensitive, reliable, and efficient alternative to radiolabeled probes for in situ hybridization of mRNA.
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