ArticleProceedings of the National Academy of Sciences of the United States of America2003
An endogenous suppressor of hairy-wing insulator separates regulatory domains in Drosophila.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 87 citations in OpenAlex.
- The N-Terminal Part ofInternational journal of molecular sciences · 2023Article
- Topological screen identifies hundreds of Cp190- and CTCF-dependentScience advances · 2023Article
- Multi-Scale Organization of theGenes · 2021Review
- An insulator blocks access to enhancers by an illegitimate promoter, preventing repression by transcriptional interference.PLoS genetics · 2021Article
- The Role of Insulators in Transgene Transvection inGenetics · 2019Article
- Transcriptionally promiscuous "blurry" promoters in Tc1/Mobile DNA · 2019Article
- Multiple interactions are involved in a highly specific association of the Mod(mdg4)-67.2 isoform with the Su(Hw) sites inOpen biology · 2017Article
- Article
- Deciphering the DNA code for the function of the Drosophila polydactyl zinc finger protein Suppressor of Hairy-wing.Nucleic acids research · 2017Article
- EAST affects the activity of Su(Hw) insulators by two different mechanisms in Drosophila melanogaster.Chromosoma · 2017Article
- Article
- Distinct Roles of Chromatin Insulator Proteins in Control of the Drosophila Bithorax Complex.Genetics · 2016Article
- An Organizational Hub of Developmentally Regulated Chromatin Loops in the Drosophila Antennapedia Complex.Molecular and cellular biology · 2015Article
- Architectural proteins, transcription, and the three-dimensional organization of the genome.FEBS letters · 2015Review
- EAST Organizes Drosophila Insulator Proteins in the Interchromosomal Nuclear Compartment and Modulates CP190 Binding to Chromatin.PloS one · 2015Article
- Sequencing and annotated analysis of full genome of Holstein breed bull.Mammalian genome : official journal of the International Mammalian Genome Society · 2014Article
- The RNA-binding protein Rumpelstiltskin antagonizes gypsy chromatin insulator function in a tissue-specific manner.Journal of cell science · 2014Article
- Study of functional activity of the 1A2 insulator and polyadenylation signal in intron of the yellow gene of Drosophila melanogaster.Doklady. Biochemistry and biophysics · 2014Article
- Surviving an identity crisis: a revised view of chromatin insulators in the genomics era.Biochimica et biophysica acta · 2014Review
- Making connections: insulators organize eukaryotic chromosomes into independent cis-regulatory networks.BioEssays : news and reviews in molecular, cellular and developmental biology · 2014Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Insulators define independent domains of gene function throughout the genome. The Drosophila gypsy insulator was isolated from the gypsy retrotransposon as a region that contains a cluster of binding sites for the Suppressor of Hairy-wing [Su(Hw)] protein. To study the effects of the gypsy insulator on gene expression within a single genomic domain, targeted gene replacement was used to exchange the endogenous yellow gene, located at cytological location 1A, with a set of gypsy-modified yellow genes. Replaced yellow genes carried a gypsy insulator positioned between the yellow promoter and either the upstream or the downstream tissue-specific enhancers. Whereas the gypsy insulator blocked the function of the upstream enhancers at the endogenous location, the downstream enhancers were not blocked. Investigation of the 1A region revealed two clustered Su(Hw)-binding sites downstream of the yellow gene, named 1A-2, that bind Su(Hw) in vivo and possess enhancer blocking function. We propose that interaction between 1A-2 and the gypsy insulator permits activation of yellow expression by enhancers in the neighboring achaete-scute complex, causing an apparent absence of the block of the downstream yellow enhancers. Based on these data, we suggest that 1A-2 is an endogenous Su(Hw) insulator that separates regulatory domains within the Drosophila genome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.