ArticleExperimental cell research2003
Unique tissue-specific level of DNA nucleotide excision repair in primary human mammary epithelial cultures.
Article in Experimental cell research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Assessment and detection of nucleotide excision repair capability in human cells: a critical review of current functional methods.Molecular biology reports · 2026Review
- Cancer-specific alterations in nuclear matrix proteins determined by multi-omics analyses of ductal carcinomaFrontiers in oncology · 2024Article
- Preliminary Evidence for a Hormetic Effect on DNA Nucleotide Excision Repair in Veterans with Gulf War Illness.Military medicine · 2020Article
- Co-administering Melatonin With an Estradiol-Progesterone Menopausal Hormone Therapy Represses Mammary Cancer Development in a Mouse Model of HER2-Positive Breast Cancer.Frontiers in oncology · 2019Article
- Canine mammary tumors as a model for human disease.Oncology letters · 2018Review
- Unscheduled DNA synthesis: the clinical and functional assay for global genomic DNA nucleotide excision repair.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Comprehensive screen of genetic variation in DNA repair pathway genes and postmenopausal breast cancer risk.Breast cancer research and treatment · 2011Article
- Nucleotide excision repair deficiency is intrinsic in sporadic stage I breast cancer.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- A modified fluorimetric host cell reactivation assay to determine the repair capacity of primary keratinocytes, melanocytes and fibroblasts.BMC biotechnology · 2010Article
- Cell-type-specific level of DNA nucleotide excision repair in primary human mammary and ovarian epithelial cell cultures.Cell and tissue research · 2008Article
- Haploinsufficiency for BRCA1 is associated with normal levels of DNA nucleotide excision repair in breast tissue and blood lymphocytes.BMC medical genetics · 2005Article
- Unscheduled DNA synthesis: a functional assay for global genomic nucleotide excision repair.Methods in molecular biology (Clifton, N.J.) · 2005Article
- Analysis of DNA repair using transfection-based host cell reactivation.Methods in molecular biology (Clifton, N.J.) · 2005Article
- Regulation and disregulation of mammalian nucleotide excision repair: a pathway to nongermline breast carcinogenesis.Photochemistry and photobiologyArticle
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
DNA repair is essential for the maintenance of genomic integrity and stability. Nucleotide excision repair (NER) is a major pathway responsible for remediation of damage caused by UV light, bulky adducts, and cross-linking agents. We now show that NER capacity is differentially expressed in human tissues. We established primary cultures of peripheral blood lymphocytes (PBLs: N = 33) and foreskin fibroblasts (FF: N = 6), as well as adult breast tissue (N = 22) using a unique culture system, and measured their NER capacity using the unscheduled DNA synthesis (UDS) functional assay. Relative to FF, primary cultures of breast cells exhibited only 24.6 +/- 2.1% of NER capacity and PBLs only 8.9 +/- 1.2%. Cells from the breast therefore have a unique and distinctive DNA repair capacity. The NER capacities of all three cell types had similar coefficients of variation in the range of 10%-15%, which should be taken into account when running controls for this contextual assay. Unlike previous studies and speculation in the field, we found that NER was not affected by cell morphology, donor age, or proliferation as measured by the S phase index. While the NER capacity of the transformed lymphoblastoid cell line TK6 was within the range of our PBL samples, the breast tumor-derived MDA MB-231 cell line was four-fold higher than normal breast tissue. These studies show that analysis of baseline DNA repair in normal human cell types is critical as a basis for evaluation of the effects of "mutator" genes as etiological factors in the development of cancer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.