ArticleThe Journal of physiology2004
Beta 2-adrenergic regulation of ciliary beat frequency in rat bronchiolar epithelium: potentiation by isosmotic cell shrinkage.
Article in The Journal of physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 42 citations in OpenAlex.
- Discovery of novel alleles in Kartagener's syndrome.Mammalian genome : official journal of the International Mammalian Genome Society · 2026Article
- Pharmacological regulation of airway ciliary motility: endogenous mediators, pharmaceutical agents, and natural compounds with a focus on ginsenoside Rd.Journal of natural medicines · 2026Review
- Physiological roles of chloride ions in bodily and cellular functions.The journal of physiological sciences : JPS · 2023Review
- Enhancement of airway ciliary beating mediated via voltage-gated CaPflugers Archiv : European journal of physiology · 2022Article
- Effects of long-acting muscarinic antagonists on promoting ciliary function in airway epithelium.BMC pulmonary medicine · 2022Article
- Influenza A virus enhances ciliary activity and mucociliary clearance via TLR3 in airway epithelium.Respiratory research · 2020Article
- Intracellular ClInternational journal of molecular sciences · 2020Review
- Airway Ciliary Beating Affected by theInternational journal of molecular sciences · 2020Article
- Ciliary beating amplitude controlled by intracellular ClPflugers Archiv : European journal of physiology · 2019Article
- Carbocisteine stimulated an increase in ciliary bend angle via a decrease in [ClPflugers Archiv : European journal of physiology · 2019Article
- Daidzein-Stimulated Increase in the Ciliary Beating Amplitude via an [ClInternational journal of molecular sciences · 2018Article
- Measurement of [ClThe journal of physiological sciences : JPS · 2018Article
- Seihai-to (TJ-90)-Induced Activation of Airway Ciliary Beatings of Mice: CaInternational journal of molecular sciences · 2018Article
- A low [CaPflugers Archiv : European journal of physiology · 2017Article
- CHD associated with syndromic diagnoses: peri-operative risk factors and early outcomes.Cardiology in the young · 2016Review
- Effects of histamine on ciliary beat frequency of ciliated cells from guinea pigs nasal mucosa.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2015Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single bronchiolar ciliary cells were isolated from rat lungs. The beta(2)-adrenergic regulation of ciliary beat frequency (CBF) was studied using video-optical microscopy. Terbutaline (a beta(2)-adrenergic agonist) increased CBF in a dose-dependent manner, and it also decreased the volume of the ciliary cells. These terbutaline actions were inhibited by a PKA inhibitor (H-89) and mimicked by forskolin, IBMX and DBcAMP. Ion transport inhibitors were used to isosmotically manipulate the volume of the terbutaline-stimulated bronchiolar ciliary cells. Amiloride (1 microM) and bumetanide (20 microM) potentiated cell shrinkage and the CBF increase, and they shifted the terbutaline dose-response curve to the lower-concentration side. Quinidine (500 microM), in contrast, increased cell volume and suppressed the CBF increase. Moreover, a KCl solution containing amiloride (1 microM) and strophanthidin (100 microM) increased cell volume and suppressed the CBF increase, and then the subsequent removal of either amiloride or strophanthidin decreased cell volume and further increased CBF. NPPB (10 microM) or glybenclamide (200 microM) had no effect on the action of terbutaline. Thus, in terbutaline-stimulated ciliary cells, cell shrinkage enhances the CBF increase; in contrast, cell swelling suppresses it. However, the results of direct manupulation of cell volume by applying osmotic stresses (hyperosmotic shrinkage or hyposmotic swelling) were the opposite of the findings of the isosmotic experiments: hyposmotic cell swelling enhanced the CBF increase, while isosmotic swelling suppressed it. These results suggest that isosmotic and non-isosmotic volume changes in terbutaline-stimulated bronchiolar ciliary cells may trigger different signalling pathways. In conclusion, terbutaline increases CBF and decreases the volume of rat bronchiolar ciliary cells via cAMP accumulation under isosmotic conditions, and the isosmotic cell shrinkage enhances the CBF increase by increasing cAMP sensitivity.
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