ArticleJournal of bacteriology2003
The ADP-ribosylating toxin, AexT, from Aeromonas salmonicida subsp. salmonicida is translocated via a type III secretion pathway.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 87 citations in OpenAlex.
- Antimicrobial resistance in aeromonads and new therapies targeting quorum sensing.Applied microbiology and biotechnology · 2024Review
- Article
- Whole genome sequence analysis ofFrontiers in microbiology · 2023Article
- Secretion Systems in Gram-Negative Bacterial Fish Pathogens.Frontiers in microbiology · 2021Review
- Type III Secretion 1 Effector Gene Diversity AmongFrontiers in cellular and infection microbiology · 2020Article
- Article
- Genome-driven evaluation and redesign of PCR tools for improving the detection of virulence-associated genes in aeromonads.PloS one · 2018Article
- Draft Genome Sequence of the Type StrainGenome announcements · 2017Article
- Cross-Talk between the Aeromonas hydrophila Type III Secretion System and Lateral Flagella System.Frontiers in microbiology · 2016Article
- Virulence Factors of Aeromonas hydrophila: In the Wake of Reclassification.Frontiers in microbiology · 2016Review
- Aeromonas salmonicida subsp. salmonicida in the light of its type-three secretion system.Microbial biotechnology · 2014Review
- Protein expression and transcription profiles of three strains of Aeromonas salmonicida ssp. salmonicida under normal and iron-limited culture conditions.Proteome science · 2014Article
- The Aeromonas salmonicida subsp. salmonicida exoproteome: determination of the complete repertoire of Type-Three Secretion System effectors and identification of other virulence factors.Proteome science · 2013Article
- Knowing your friends: invertebrate innate immunity fosters beneficial bacterial symbioses.Nature reviews. Microbiology · 2012Review
- An insertion sequence-dependent plasmid rearrangement in Aeromonas salmonicida causes the loss of the type three secretion system.PloS one · 2012Article
- The main Aeromonas pathogenic factors.ISRN microbiology · 2012Article
- Article
- Complex evolutionary history of the Aeromonas veronii group revealed by host interaction and DNA sequence data.PloS one · 2011Article
- Unraveling the mechanism of action of a new type III secretion system effector AexU from Aeromonas hydrophila.Microbial pathogenesis · 2010Article
- N-acylhomoserine lactones involved in quorum sensing control the type VI secretion system, biofilm formation, protease production, and in vivo virulence in a clinical isolate of Aeromonas hydrophila.Microbiology (Reading, England) · 2009Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
AexT is an extracellular ADP ribosyltransferase produced by the fish pathogen Aeromonas salmonicida subsp. salmonicida. The protein is secreted by the bacterium via a recently identified type III secretion system. In this study, we have identified a further 12 open reading frames that possess high homology to genes encoding both structural and regulatory components of the Yersinia type III secretion apparatus. Using marker replacement mutagenesis of aopB, the A. salmonicida subsp. salmonicida homologue of yopB in Yersinia, we demonstrate that the bacterium translocates the AexT toxin directly into the cytosol of cultured fish cells via this type III secretion pathway. An acrV mutant of A. salmonicida subsp. salmonicida displays a calcium-blind phenotype, expressing and secreting significant amounts of AexT even in the presence of CaCl2 concentrations as high as 10 mM. This acrV mutant is also unable to translocate AexT into the cytosol of fish cells, indicating AcrV is involved in the translocation process. Inactivation of either the aopB or acrV gene in A. salmonicida subsp. salmonicida (resulting in an inability to translocate AexT) is accompanied by a loss of cytotoxicity that can be restored by trans complementation. Finally, we present data indicating that preincubation of the wild-type bacteria with antibodies directed against recombinant AcrV-His protein provides fish cells protection against the toxic effects of the bacterium.
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