ArticleThe EMBO journal2003
Mutations occur in the Ig Smu region but rarely in Sgamma regions prior to class switch recombination.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- APE2 Promotes AID-Dependent Somatic Hypermutation in Primary B Cell Cultures That Is Suppressed by APE1.Journal of immunology (Baltimore, Md. : 1950) · 2023Article
- Loop extrusion promotes an alternate pathway for isotype switching.Cell reports · 2021Article
- Immunoglobulin Class Switch Recombination Is Initiated by Rare Cytosine Deamination Events at Switch Regions.Molecular and cellular biology · 2020Article
- Article
- CCCTC-Binding Factor Locks Premature IgH Germline Transcription and Restrains Class Switch Recombination.Frontiers in immunology · 2017Article
- Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian cells.Nature methods · 2016Article
- A Damage-Independent Role for 53BP1 that Impacts Break Order and Igh Architecture during Class Switch Recombination.Cell reports · 2016Article
- Nbs1 ChIP-Seq Identifies Off-Target DNA Double-Strand Breaks Induced by AID in Activated Splenic B Cells.PLoS genetics · 2015Article
- Individual substitution mutations in the AID C terminus that ablate IgH class switch recombination.PloS one · 2015Article
- IgH chain class switch recombination: mechanism and regulation.Journal of immunology (Baltimore, Md. : 1950) · 2014Review
- ATM increases activation-induced cytidine deaminase activity at downstream S regions during class-switch recombination.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- B cell-specific deficiencies in mTOR limit humoral immune responses.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Three-dimensional architecture of the IgH locus facilitates class switch recombination.Annals of the New York Academy of Sciences · 2012Article
- Reprogramming IgH isotype-switched B cells to functional-grade induced pluripotent stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- AIDing antibody diversity by error-prone mismatch repair.Seminars in immunology · 2012Review
- Negative supercoiling creates single-stranded patches of DNA that are substrates for AID-mediated mutagenesis.PLoS genetics · 2012Article
- Epigenetic tethering of AID to the donor switch region during immunoglobulin class switch recombination.The Journal of experimental medicine · 2011Article
- Impaired induction of DNA lesions during immunoglobulin class-switch recombination in humans influences end-joining repair.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Downstream class switching leads to IgE antibody production by B lymphocytes lacking IgM switch regions.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Alternative end-joining catalyzes robust IgH locus deletions and translocations in the combined absence of ligase 4 and Ku70.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Nucleotide substitutions are found in recombined Ig switch (S) regions and also in unrecombined (germline, GL) Smicro segments in activated splenic B cells. Herein we examine whether mutations are also introduced into the downstream acceptor S regions prior to switch recombination, but find very few mutations in GL Sgamma3 and Sgamma1 regions in activated B cells. These data suggest that switch recombination initiates in the Smicro segment and secondarily involves the downstream acceptor S region. Furthermore, the pattern and specificity of mutations in GL and recombined Smicro segments differ, suggesting different repair mechanisms. Mutations in recombined Smicro regions show a strong bias toward G/C base pairs and WRCY/RGYW hotspots, whereas mutations introduced into the GL Smicro do not. Additionally, induction conditions affect mutation specificity within the GL Smicro segment. Mutations are most frequent near the S-S junctions and decrease rapidly with distance from the junction. Finally, we find that mice expressing a transgene for terminal deoxynucleotidyl transferase (TdT) have nucleotide insertions at S-S junctions, indicating that the recombining DNA ends are accessible to end-processing enzyme activities.
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