ArticleThe EMBO journal2003
Rab5-mediated endosome-endosome fusion regulates hemoglobin endocytosis in Leishmania donovani.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 64 citations in OpenAlex.
- Heme and immunity: The heme oxygenase dichotomy.Journal of inorganic biochemistry · 2025Review
- Evolutionary duplication of the leishmanial adaptor protein α-SNAP plays a role in its pathogenicity.The Journal of biological chemistry · 2025Article
- Leishmania highjack host lipid body for its proliferation in macrophages by overexpressing host Rab18 and TRAPPC9 by downregulating miR-1914-3p expression.PLoS pathogens · 2024Article
- Access and utilization of host-derived iron by Leishmania parasites.Journal of biochemistry · 2023Review
- Hemoglobin uptake and utilization by human protozoan parasites: a review.Frontiers in cellular and infection microbiology · 2023Review
- New Insights on Heme Uptake inInternational journal of molecular sciences · 2022Article
- Hemoglobin Endocytosis and Intracellular Trafficking: A Novel Way of Heme Acquisition byPathogens (Basel, Switzerland) · 2022Review
- Leishmania heme uptake involves LmFLVCRb, a novel porphyrin transporter essential for the parasite.Cellular and molecular life sciences : CMLS · 2020Article
- Iron and Heme Metabolism at the Leishmania-Host Interface.Trends in parasitology · 2020Review
- Endocytosis and Exocytosis inFrontiers in cellular and infection microbiology · 2020Article
- Functional aspects, phenotypic heterogeneity, and tissue immune response of macrophages in infectious diseases.Infection and drug resistance · 2019Article
- Structural and Biophysical Characterization of Rab5a from Leishmania Donovani.Biophysical journal · 2018Article
- GTPase Sar1 regulates the trafficking and secretion of the virulence factor gp63 inThe Journal of biological chemistry · 2017Article
- Leishmania donovani resides in modified early endosomes by upregulating Rab5a expression via the downregulation of miR-494.PLoS pathogens · 2017Article
- Leishmania major large RAB GTPase is highly immunogenic in individuals immune to cutaneous and visceral leishmaniasis.Parasites & vectors · 2017Article
- Single GDP-dissociation Inhibitor Protein regulates endocytic and secretory pathways in Leishmania.Scientific reports · 2016Article
- Rab5 Isoforms Specifically Regulate Different Modes of Endocytosis in Leishmania.The Journal of biological chemistry · 2016Article
- LmABCB3, an atypical mitochondrial ABC transporter essential for Leishmania major virulence, acts in heme and cytosolic iron/sulfur clusters biogenesis.Parasites & vectors · 2016Article
- Functional Characterization of Monomeric GTPase Rab1 in the Secretory Pathway of Leishmania.The Journal of biological chemistry · 2015Article
- Evolutionary comparison of prenylation pathway in kinetoplastid Leishmania and its sister Leptomonas.BMC evolutionary biology · 2015Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To understand the trafficking of endocytosed hemoglobin (Hb) in Leishmania, we investigated the characteristics of in vitro fusion between endosomes containing biotinylated Hb (BHb) and avidin-horseradish peroxidase (AHRP). We showed that early endosome fusion in Leishmania is temperature and cytosol dependent and is inhibited by ATP depletion, ATPgammaS, GTPgammaS and N-ethylmaleimide treatment. The Rab5 homolog from Leishmania donovani, LdRab5, was cloned and expressed. Our results showed that homotypic fusion between the early endosomes in Leishmania is Rab5 dependent. Early endosomes containing BHb fused efficiently with late endosomes in a process regulated by Rab7, whereas no fusion between early and late endosomes was detected using fluid phase markers. Pre-treatment of early endosomes containing BHb with monoclonal antibody specific for the C-terminus of the Hb receptor (HbR) or the addition of the C-terminal cytoplasmic fragment of the HbR specifically inhibited the fusion with late endosomes, suggesting that signal(s) mediated through the HbR cytoplasmic tail promotes the fusion of early endosomes containing Hb with late endosomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.