ArticleMolecular and cellular biology2003
Retinoblastoma tumor suppressor: analyses of dynamic behavior in living cells reveal multiple modes of regulation.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 35 citations in OpenAlex.
- Recruitment of Pontin/Reptin by E2f1 amplifies E2f transcriptional response during cancer progression.Nature communications · 2015Article
- Nucleolar repression facilitates initiation and maintenance of senescence.Cell cycle (Georgetown, Tex.) · 2015Article
- Cbx2 stably associates with mitotic chromosomes via a PRC2- or PRC1-independent mechanism and is needed for recruiting PRC1 complex to mitotic chromosomes.Molecular biology of the cell · 2014Article
- Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression.The Journal of experimental medicine · 2013Article
- The RB family is required for the self-renewal and survival of human embryonic stem cells.Nature communications · 2012Article
- FERM domain phosphoinositide binding targets merlin to the membrane and is essential for its growth-suppressive function.Molecular and cellular biology · 2011Article
- Dynamic as well as stable protein interactions contribute to genome function and maintenance.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
- Identification and functional analysis of NOL7 nuclear and nucleolar localization signals.BMC cell biology · 2010Article
- Retinoblastoma/p107/p130 pocket proteins: protein dynamics and interactions with target gene promoters.The Journal of biological chemistry · 2009Article
- Protein-protein interaction analysis by C-terminally specific fluorescence labeling and fluorescence cross-correlation spectroscopy.Nucleic acids research · 2006Article
- Partner-regulated interaction of IFN regulatory factor 8 with chromatin visualized in live macrophages.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Dynamic targeting of the replication machinery to sites of DNA damage.The Journal of cell biology · 2004Article
- RB reversibly inhibits DNA replication via two temporally distinct mechanisms.Molecular and cellular biology · 2004Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The retinoblastoma tumor suppressor, RB, assembles multiprotein complexes to mediate cell cycle inhibition. Although many RB binding partners have been suggested to underlie these functions, the validity of these interactions on the behavior of RB complexes in living cells has not been investigated. Here, we studied the dynamic behavior of RB by using green fluorescent protein-RB fusion proteins. Although these proteins were universally nuclear, phosphorylation or oncoprotein binding mediated their active exclusion from the nucleolus. In vivo imaging approaches revealed that RB exists in dynamic equilibrium between a highly mobile and a slower diffusing species, and genetic lesions associated with tumorigenesis increased the fraction of RB in a highly mobile state. The RB complexes dictating cell cycle arrest were surprisingly dynamic and harbored a relatively short residence time on chromatin. In contrast, this rapid exchange was attenuated in cells that are hypersensitive to RB, suggesting that responsiveness may inversely correlate with mobility. The stability of RB dynamics within the cell was additionally modified by the presence and function of critical corepressors. Last, the RB-assembled complexes present in living cells were primarily associated with E2F binding sites in chromatin. In contrast to RB, E2F1 consistently maintained a stable association with E2F sites regardless of cell type. Together, these results elucidate the kinetic framework of RB tumor suppressor action in transcriptional repression and cell cycle regulation.
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