ArticleMolecular and cellular biology2003
A human globin enhancer causes both discrete and widespread alterations in chromatin structure.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 42 citations in OpenAlex.
- Genome-wide measurement of local nucleosome array regularity and spacing by nanopore sequencing.Nature structural & molecular biology · 2018Article
- Differential contribution of cis-regulatory elements to higher order chromatin structure and expression of the CFTR locus.Nucleic acids research · 2016Article
- An H4K16 histone acetyltransferase mediates decondensation of the X chromosome inEpigenetics & chromatin · 2016Article
- The emerging roles of eRNAs in transcriptional regulatory networks.RNA biology · 2014Article
- CTCF demarcates chicken embryonic α-globin gene autonomous silencing and contributes to adult stage-specific gene expression.Epigenetics · 2013Article
- Altered expression of nuclear and cytoplasmic histone H1 in pulmonary artery and pulmonary artery smooth muscle cells in patients with IPAH.Pulmonary circulation · 2012Article
- The distinctive roles of erythroid specific activator GATA-1 and NF-E2 in transcription of the human fetal γ-globin genes.Nucleic acids research · 2011Article
- Article
- Dissecting the regulatory switches of development: lessons from enhancer evolution in Drosophila.Development (Cambridge, England) · 2010Review
- Differential requirement of a distal regulatory region for pre-initiation complex formation at globin gene promoters.Nucleic acids research · 2009Article
- The apolipoprotein CIII enhancer regulates both extensive histone modification and intergenic transcription of human apolipoprotein AI/CIII/AIV genes but not apolipoprotein AV.The Journal of biological chemistry · 2008Article
- The role of transcriptional activator GATA-1 at human beta-globin HS2.Nucleic acids research · 2008Article
- A positive role for NLI/Ldb1 in long-range beta-globin locus control region function.Molecular cell · 2007Article
- Linear distance from the locus control region determines epsilon-globin transcriptional activity.Molecular and cellular biology · 2007Article
- A modular enhancer is differentially regulated by GATA and NFAT elements that direct different tissue-specific patterns of nucleosome positioning and inducible chromatin remodeling.Molecular and cellular biology · 2007Article
- Beta-globin intergenic transcription and histone acetylation dependent on an enhancer.Molecular and cellular biology · 2007Article
- Nucleosome and transcription activator antagonism at human beta-globin locus control region DNase I hypersensitive sites.Nucleic acids research · 2007Article
- Review
- Role of linker histone in chromatin structure and function: H1 stoichiometry and nucleosome repeat length.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2006Review
- Heterogeneous nuclear ribonucleoprotein C1/C2, MeCP1, and SWI/SNF form a chromatin remodeling complex at the beta-globin locus control region.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
Gene activation requires alteration of chromatin structure to facilitate active transcription complex formation at a gene promoter. Nucleosome remodeling complexes and histone modifying complexes each play unique and interdependent roles in bringing about these changes. The role of distant enhancers in these structural alterations is not well understood. We studied nucleosome remodeling and covalent histone modification mediated by the beta-globin locus control region HS2 enhancer at nucleosome-level resolution throughout a 5.5-kb globin gene model locus in vivo in K562 cells. We compared the transcriptionally active locus to one in which HS2 was inactivated by mutations in the core NF-E2 sites. In contrast to inactive templates, nucleosomes were mobilized in discrete areas of the active locus, including the HS2 core and the proximal promoter. Large differences in restriction enzyme accessibility between the active and inactive templates were limited to the regions of nucleosome mobilization, which subsumed the DNase I hypersensitive sites. In contrast to this discrete pattern, histone H3 and H4 acetylation and H3 K4 methylation were elevated across the entire active locus, accompanied by depletion of linker histone H1. The coding region of the gene differed from the regulatory regions, demonstrating both nucleosome mobilization and histone hyperacetylation, but lacked differences in restriction enzyme accessibility between transcriptionally active and inactive genes. Thus, although the histone modification pattern we observe is consistent with the spreading of histone modifying activity from the distant enhancer, the pattern of nucleosome mobilization is more compatible with direct contact between an enhancer and promoter.
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