ArticleProceedings of the National Academy of Sciences of the United States of America2003
Identification and characterization of a cyclooxygenase-like enzyme from Entamoeba histolytica.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 64 citations in OpenAlex.
- Article
- Exploration of the Binding Site of Arachidonic Acid in gp63 ofPathogens (Basel, Switzerland) · 2024Article
- Extracellular Vesicles Secreted byMicroorganisms · 2023Article
- Amoebic liver abscess in a COVID-19 patient: a case report.BMC infectious diseases · 2021Article
- Functional Characterization of an Interferon Gamma Receptor-Like Protein on Entamoeba histolytica.Infection and immunity · 2019Article
- Review
- Co-opting oxylipin signals in microbial disease.Cellular microbiology · 2019Review
- The Role of Ion Transporters in the Pathophysiology of Infectious Diarrhea.Cellular and molecular gastroenterology and hepatology · 2018Review
- Article
- Entamoeba histolytica: Host parasite interactions at the colonic epithelium.Tissue barriers · 2017Article
- Entamoeba histolytica Cysteine Proteinase 5 Evokes Mucin Exocytosis from Colonic Goblet Cells via αvβ3 Integrin.PLoS pathogens · 2016Article
- Immune Evasion Mechanisms of Entamoeba histolytica: Progression to Disease.Frontiers in microbiology · 2015Review
- Peroxynitrite and peroxiredoxin in the pathogenesis of experimental amebic liver abscess.BioMed research international · 2014Review
- Host immune response to intestinal amebiasis.PLoS pathogens · 2013Review
- The dynamic interdependence of amebiasis, innate immunity, and undernutrition.Seminars in immunopathology · 2012Review
- Transferrin regulates mRNA levels of a gene involved in iron utilization in Entamoeba histolytica.Molecular biology reports · 2012Article
- Prostaglandin E2 produced by Entamoeba histolytica signals via EP4 receptor and alters claudin-4 to increase ion permeability of tight junctions.The American journal of pathology · 2011Article
- Insulin/FOXO signaling regulates ovarian prostaglandins critical for reproduction.Developmental cell · 2010Article
- Endocannabinoids inhibit the growth of free-living amoebae.Antimicrobial agents and chemotherapy · 2010Article
- Infectious diarrhea: Cellular and molecular mechanisms.Gut microbes · 2010Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The intestinal protozoan parasite Entamoeba histolytica remains a significant cause of morbidity and mortality worldwide. However, almost nothing is known about the molecules secreted by the parasite that modulate host immune responses or epithelial barrier function in the colon. Herein, we describe the isolation and characterization of a cyclooxygenase (COX)-like enzyme in E. histolytica that is responsible for the biosynthesis of prostaglandin (PG)E2. PGE2 produced by ameba was constitutive but highly dependent on exogenous arachidonic acid substrate. COX-like activity and the immunoreactive protein were localized to the nuclear fraction of E. histolytica. The COX-like protein (72 kDa) was microsequenced and cloned by reverse transcriptase PCR. Ameba COX showed little homology with COX-1/2 enzymes from different species at the nucleotide and amino acid levels. Surprisingly, the arachidonate-binding domain and heme-coordinating and catalytic sites, which are conserved in other species, were absent in ameba. Ameba COX expressed in Escherichia coli demonstrated COX-like enzyme activity in vitro by converting arachidonic acid into PGE2 but not into PGD2 or PGF2alpha. COX activity was inhibited with 1 mM aspirin but not with indomethacin or COX-1/2-specific inhibitors. Taken together, these studies reveal that E. histolytica produces PGE2, by means of a previously undescribed ancestral COX-like enzyme, which could play a major role in pathogenesis and immune evasion.
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