ArticleJournal of virology2003
The pseudorabies virus Us2 protein, a virion tegument component, is prenylated in infected cells.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 33 citations in OpenAlex.
- Duck plague virus US2 promotes p62-mediated autophagic degradation of RIG-I to suppress antiviral signaling.Poultry science · 2026Article
- The prenylated pUS2 protein of pseudorabies virus contributes to phosphorylation of connexin 43 and suppression of gap junctional intercellular communication.Journal of virology · 2026Article
- Duck plague virus LORF2 utilizes RNF34 to inhibit antiviral innate immunity by ubiquitination and degradation of IRF7.PLoS pathogens · 2026Article
- Pseudorabies virus tegument protein US2 antagonizes antiviral innate immunity by targeting cGAS-STING signaling pathway.Frontiers in immunology · 2024Article
- Proteomic Comparison of Three Wild-Type Pseudorabies Virus Strains and the Attenuated Bartha Strain Reveals Reduced Incorporation of Several Tegument Proteins in Bartha Virions.Journal of virology · 2022Article
- The Attenuated Pseudorabies Virus Vaccine Strain Bartha Hyperactivates Plasmacytoid Dendritic Cells by Generating Large Amounts of Cell-Free Virus in Infected Epithelial Cells.Journal of virology · 2022Article
- Alphaherpesvirus gB Homologs Are Targeted to Extracellular Vesicles, but They Differentially Affect MHC Class II Molecules.Viruses · 2020Article
- Deletion of pseudorabies virus US2 gene enhances viral titers in a porcine cerebral cortex primary culture system.Virus genes · 2018Article
- Comparison of Pathogenicity-Related Genes in the Current Pseudorabies Virus Outbreak in China.Scientific reports · 2017Article
- Plant polyisoprenoids and control of cholesterol level.Archivum immunologiae et therapiae experimentalis · 2014Review
- Glycoproteins gE and gI are required for efficient KIF1A-dependent anterograde axonal transport of alphaherpesvirus particles in neurons.Journal of virology · 2013Article
- The Us2 gene product of herpes simplex virus 2 is a membrane-associated ubiquitin-interacting protein.Journal of virology · 2013Article
- The transcription factor STAT-1 couples macrophage synthesis of 25-hydroxycholesterol to the interferon antiviral response.Immunity · 2013Article
- Analysis of viral and cellular factors influencing herpesvirus-induced nuclear envelope breakdown.Journal of virology · 2012Article
- Proteomic characterization of pseudorabies virus extracellular virions.Journal of virology · 2011Article
- Pseudorabies virus tegument protein Us2 recruits the mitogen-activated protein kinase extracellular-regulated kinase (ERK) to membranes through interaction with the ERK common docking domain.Journal of virology · 2010Article
- Further analysis of Marek's disease virus horizontal transmission confirms that U(L)44 (gC) and U(L)13 protein kinase activity are essential, while U(S)2 is nonessential.Journal of virology · 2010Article
- Comparison of the pseudorabies virus Us9 protein with homologs from other veterinary and human alphaherpesviruses.Journal of virology · 2009Article
- Molecular analysis of US10, S3, and US2 in duck enteritis virus.Virus genes · 2009Article
- Targeting of pseudorabies virus structural proteins to axons requires association of the viral Us9 protein with lipid rafts.PLoS pathogens · 2008Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The Us2 gene is conserved among alphaherpesviruses, but its function is not known. We demonstrate here that the pseudorabies virus (PRV) Us2 protein is synthesized early after infection and localizes to cytoplasmic vesicles and to the plasma membrane, despite the lack of a recognizable signal sequence or membrane-spanning domain. Us2 protein is also packaged as part of the tegument of mature virions. The Us2 carboxy-terminal four amino acids comprise a CAAX motif, a well-characterized signal for protein prenylation. Treatment of infected cells with lovastatin, a drug that disrupts protein prenylation, changed the relative electrophoretic mobility of Us2 in sodium dodecyl sulfate-polyacrylamide gels. In addition, lovastatin treatment caused a dramatic relocalization of Us2 to cytoplasmic punctate structures associated with microtubules, which appeared to concentrate over the microtubule organizing center. When the CAAX motif was changed to GAAX and the mutant protein was synthesized from an expression plasmid, it concentrated in punctate cytoplasmic structures reminiscent of Us2 localization in infected cells treated with lovastatin. We suggest that prenylation of PRV Us2 protein is required for proper membrane association. Curiously, the Us2 protein isolated from purified virions does not appear to be prenylated. This is the first report to describe the prenylation of an alphaherpesvirus protein.
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