ArticleThe Journal of cell biology2003
The Rab8 GTPase selectively regulates AP-1B-dependent basolateral transport in polarized Madin-Darby canine kidney cells.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers.
What it found
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Who cites it
108 citing papers in PubMed, 214 citations in OpenAlex.
- A conformation-dependent hydrophobic degron determines Rab9a-mediated vesicular trafficking.The Journal of biological chemistry · 2026Article
- RAB-10 cooperates with EHBP-1 to capture vesicular carriers during post-Golgi exocytic trafficking.The Journal of cell biology · 2025Article
- Endosomal traffic disorders: a driving force behind neurodegenerative diseases.Translational neurodegeneration · 2024Review
- Ehbp1 orchestrates orderly sorting of Wnt/Wingless to the basolateral and apical cell membranes.EMBO reports · 2024Article
- Viral and cellular determinants of polarized trafficking of viral envelope proteins from insect-specific and insect-vectored viruses in insect midgut and salivary gland cells.Journal of virology · 2024Article
- Receptor-mediated internalization promotes increased endosome size and number in a RAB4- and RAB5-dependent manner.European journal of cell biology · 2023Article
- A Brucella effector modulates the Arf6-Rab8a GTPase cascade to promote intravacuolar replication.The EMBO journal · 2021Article
- MICAL-L1 is required for cargo protein delivery to the cell surface.Biology open · 2021Article
- The Clathrin adaptor AP-1 and Stratum act in parallel pathways to control Notch activation inDevelopment (Cambridge, England) · 2021Article
- AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia.Frontiers in cell and developmental biology · 2021Article
- Terminal web and vesicle trafficking proteins mediate nematode single-cell tubulogenesis.The Journal of cell biology · 2020Article
- GRAF2, WDR44, and MICAL1 mediate Rab8/10/11-dependent export of E-cadherin, MMP14, and CFTR ΔF508.The Journal of cell biology · 2020Article
- Rab8a is involved in membrane trafficking of Kir6.2 in the MIN6 insulinoma cell line.Pflugers Archiv : European journal of physiology · 2019Article
- Cytoplasmic control of Rab family small GTPases through BAG6.EMBO reports · 2019Article
- Rab27a facilitates human parainfluenza virus type 2 growth by promoting cell surface transport of envelope proteins.Medical microbiology and immunology · 2018Article
- Optineurin: A Coordinator of Membrane-Associated Cargo Trafficking and Autophagy.Frontiers in immunology · 2018Review
- Regulation of NOTCH signaling by RAB7 and RAB8 requires carboxyl methylation by ICMT.The Journal of cell biology · 2017Article
- Polarized Exocytosis.Cold Spring Harbor perspectives in biology · 2017Review
- Regulation of Transporters and Channels by Membrane-Trafficking Complexes in Epithelial Cells.Cold Spring Harbor perspectives in biology · 2017Review
- Article
48 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The AP-1B clathrin adaptor complex plays a key role in the recognition and intracellular transport of many membrane proteins destined for the basolateral surface of epithelial cells. However, little is known about other components that act in conjunction with AP-1B. We found that the Rab8 GTPase is one such component. Expression of a constitutively activated GTP hydrolysis mutant selectively inhibited basolateral (but not apical) transport of newly synthesized membrane proteins. Moreover, the effects were limited to AP-1B-dependent basolateral cargo; basolateral transport of proteins containing dileucine targeting motifs that do not interact with AP-1B were targeted normally despite overexpression of mutant Rab8. Similar results were obtained for a dominant-negative allele of the Rho GTPase Cdc42, previously implicated in basolateral transport but now shown to be selective for the AP-1B pathway. Rab8-GFP was localized to membranes in the TGN-recycling endosome, together with AP-1B complexes and the closely related but ubiquitously expressed AP-1A complex. However, expression of active Rab8 caused a selective dissociation of AP-1B complexes, reflecting the specificity of Rab8 for AP-1B-dependent transport.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.