ArticleThe Journal of cell biology2003
Clathrin promotes incorporation of cargo into coated pits by activation of the AP2 adaptor micro2 kinase.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 64 citations in OpenAlex.
- Phosphorylation of ADAPTOR PROTEIN-2 μ-adaptin by ADAPTOR-ASSOCIATED KINASE1 regulates the tropic growth of Arabidopsis roots.The Plant cell · 2023Article
- Rab GTPases: The principal players in crafting the regulatory landscape of endosomal trafficking.Computational and structural biotechnology journal · 2022Review
- Conformational regulation of AP1 and AP2 clathrin adaptor complexes.Traffic (Copenhagen, Denmark) · 2019Review
- Article
- Temporal Ordering in Endocytic Clathrin-Coated Vesicle Formation via AP2 Phosphorylation.Developmental cell · 2019Article
- Article
- Regulation of clathrin-mediated endocytosis by hierarchical allosteric activation of AP2.The Journal of cell biology · 2017Article
- Watching real-time endocytosis in living cells.The Journal of cell biology · 2017Article
- Rab GTPases: The Key Players in the Molecular Pathway of Parkinson's Disease.Frontiers in cellular neuroscience · 2017Review
- Inhibition of AAK1 Kinase as a Novel Therapeutic Approach to Treat Neuropathic Pain.The Journal of pharmacology and experimental therapeutics · 2016Article
- Family-wide Structural Analysis of Human Numb-Associated Protein Kinases.Structure (London, England : 1993) · 2016Article
- Article
- SDF-1 chemokine signalling modulates the apoptotic responses to iron deprivation of clathrin-depleted DT40 cells.PloS one · 2014Article
- A C-terminal tyrosine-based motif in the bile salt export pump directs clathrin-dependent endocytosis.Hepatology (Baltimore, Md.) · 2012Article
- The adaptor-associated kinase 1, AAK1, is a positive regulator of the Notch pathway.The Journal of biological chemistry · 2011Article
- Role of Rab GTPases in membrane traffic and cell physiology.Physiological reviews · 2011Review
- Tickets to ride: selecting cargo for clathrin-regulated internalization.Nature reviews. Molecular cell biology · 2009Review
- Coordinated regulation of AP2 uncoating from clathrin-coated vesicles by rab5 and hRME-6.The Journal of cell biology · 2008Article
- Article
- The clathrin-binding motif and the J-domain of Drosophila Auxilin are essential for facilitating Notch ligand endocytosis.BMC developmental biology · 2008Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endocytic cargo such as the transferrin receptor is incorporated into clathrin-coated pits by associating, via tyrosine-based motifs, with the AP2 complex. Cargo-AP2 interactions occur via the mu2 subunit of AP2, which needs to be phosphorylated for endocytosis to occur. The most likely role for mu2 phosphorylation is in cargo recruitment because mu2 phosphorylation enhances its binding to internalization motifs. Here, we investigate the control of mu2 phosphorylation. We identify clathrin as a specific activator of the mu2 kinase and, in permeabilized cells, we show that ligand sequestration, driven by exogenous clathrin, results in elevated levels of mu2 phosphorylation. Furthermore, we show that AP2 containing phospho-mu2 is mainly associated with assembled clathrin in vivo, and that the level of phospho-mu2 is strongly reduced in a chicken B cell line depleted of clathrin heavy chain. Our results imply a central role for clathrin in the regulation of cargo selection via the modulation of phospho-mu2 levels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.