ArticleBiophysical journal2003
Quantitative analysis of spontaneous mitochondrial depolarizations.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
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- Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties.Nature cell biology · 2025Article
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- Fluorescence imaging detection of nanodomain redox signaling events at organellar contacts.STAR protocols · 2022Article
- Mitochondrial CaInternational journal of molecular sciences · 2022Review
- IP-Se-06, a Selenylated Imidazo[1,2-Oxidative medicine and cellular longevity · 2022Article
- Role of Calcium/Calcineurin Signalling in Regulating Intracellular Reactive Oxygen Species Homeostasis inGenes · 2021Article
- Fission and fusion machineries converge at ER contact sites to regulate mitochondrial morphology.The Journal of cell biology · 2020Article
- Temporal integration of mitochondrial stress signals by the PINK1:Parkin pathway.BMC molecular and cell biology · 2019Article
- Mitochondrial membrane potential.Analytical biochemistry · 2018Review
- Cardiac metabolic effects of KFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Transitional correlation between inner-membrane potential and ATP levels of neuronal mitochondria.Scientific reports · 2018Article
- Mitochondrial Heterogeneity.Frontiers in genetics · 2018Review
- Biogreen Synthesis of Carbon Dots for Biotechnology and Nanomedicine Applications.Nano-micro letters · 2018Review
- Live calcium and mitochondrial imaging in the enteric nervous system of Parkinson patients and controls.eLife · 2017Article
- Functional Implications of Cardiac Mitochondria Clustering.Advances in experimental medicine and biology · 2017Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Spontaneous transient depolarizations in mitochondrial membrane potential (DeltaPsi(m)), mitochondrial flickers, have been observed in isolated mitochondria and intact cells using the fluorescent probe, tetramethylrhodamine ethyl ester (TMRE). In theory, the ratio of [TMRE] in cytosol and mitochondrion allows DeltaPsi(m) to be calculated with the Nernst equation, but this has proven difficult in practice due to fluorescence quenching and binding of dye to mitochondrial membranes. We developed a new method to determine the amplitude of flickers in terms of millivolts of depolarization. TMRE fluorescence was monitored using high-speed, high-sensitivity three-dimensional imaging to track individual mitochondria in freshly dissociated smooth muscle cells. Resting mitochondrial fluorescence, an exponential function of resting DeltaPsi(m), varied among mitochondria and was approximately normally distributed. Spontaneous changes in mitochondrial fluorescence, indicating depolarizations and repolarizations in DeltaPsi(m), were observed. The depolarizations were reversible and did not result in permanent depolarization of the mitochondria. The magnitude of the flickers ranged from <10 mV to >100 mV with a mean of 17.6 +/- 1.0 mV (n = 360) and a distribution skewed to smaller values. Nearly all mitochondria flickered, and they did so independently of one another, indicating that mitochondria function as independent units in the myocytes employed here.
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Registered trials
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