ArticleBritish journal of pharmacology2003
Possible role of the protein kinase C/CPI-17 pathway in the augmented contraction of human myometrium after gestation.
Article in British journal of pharmacology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 63 citations in OpenAlex.
- Proteomic Profiles and Protein Network Analysis of Primary Human Leukocytes Revealed Possible Clearance Biomarkers for Staphylococcus aureus Infection.Current microbiology · 2023Article
- Characterization of Contractile Machinery of Vascular Smooth Muscles in Hypertension.Life (Basel, Switzerland) · 2021Review
- Article
- Differential Regulation of Myosin Regulatory Light Chain Phosphorylation by Protein Kinase C Isozymes in Human Uterine Myocytes.Reproductive sciences (Thousand Oaks, Calif.) · 2019Article
- Implications of immune-inflammatory responses in smooth muscle dysfunction and disease.Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi · 2019Article
- S-adenosyl methionine regulates calcium channels and inhibits uterine smooth muscle contraction in rats with infectious premature delivery through the transient receptor protein 3/protein kinase Cβ/C-kinase-activated protein phosphatase-1 inhibitor of 17 kDa signaling pathway.Experimental and therapeutic medicine · 2018Article
- Preventative effects of a HIF inhibitor, 17-DMAG, on partial bladder outlet obstruction-induced bladder dysfunction.American journal of physiology. Renal physiology · 2017Article
- Ryanodine receptor type 3 does not contribute to contractions in the mouse myometrium regardless of pregnancy.Pflugers Archiv : European journal of physiology · 2017Article
- Mechanisms underlying the pathogenesis of hyper-contractility of bronchial smooth muscle in allergic asthma.Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi · 2017Review
- Protein kinase C modulates frequency of micturition and non-voiding contractions in the urinary bladder via neuronal and myogenic mechanisms.BMC urology · 2015Article
- Spontaneous and evoked contractions are regulated by PKC-mediated signaling in detrusor smooth muscle: involvement of BK channels.American journal of physiology. Renal physiology · 2013Article
- A close look at the contraction and relaxation of the myometrium; the role of calcium.Journal of the Turkish German Gynecological Association · 2013Review
- Endogenous inhibitor proteins that connect Ser/Thr kinases and phosphatases in cell signaling.IUBMB life · 2012Review
- Reciprocal regulation controlling the expression of CPI-17, a specific inhibitor protein for the myosin light chain phosphatase in vascular smooth muscle cells.American journal of physiology. Cell physiology · 2012Article
- Phasic contractions of isolated human myometrium are associated with Rho-kinase (ROCK)-dependent phosphorylation of myosin phosphatase-targeting subunit (MYPT1).Molecular human reproduction · 2012Article
- Deletion of SM-B, the high ATPase isoform of myosin, upregulates the PKC-mediated signal transduction pathway in murine urinary bladder smooth muscle.American journal of physiology. Renal physiology · 2009Article
- Regulation of the uterine contractile apparatus and cytoskeleton.Seminars in cell & developmental biology · 2007Review
- Protein kinase C and human uterine contractility.BMC pregnancy and childbirth · 2007Review
- Expression of scaffolding, signalling and contractile-filament proteins in human myometria: effects of pregnancy and labour.Journal of cellular and molecular medicineArticle
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Activation of protein kinase C (PKC) by phorbol 12,13-dibutylate (PDBu, 1 microm) induced sustained contractions with no increase in [Ca2+]i in nonpregnant and pregnant human myometria. The contractile effects of PDBu in pregnant myometrium were much greater than those in nonpregnant myometrium, and the contractions in pregnant myometrium were accompanied by an increase in myosin light chain (MLC) phosphorylation at Ser19. 2. The contraction induced by PDBu in pregnant myometrium was inhibited by the inhibitors of conventional PKC isoforms, bisindolylmaleimides and indolocarbazole, such as Go6976, Go6983, and Go6850 (1 microM). LY333531 (1 microM), a specific inhibitor of PKC beta, also inhibited the PDBu-induced contraction in the pregnant myometrium. 3. In the pregnant myometrium permeabilized with alpha-toxin, PDBu increased the contractions induced at fixed Ca2+ concentration (0.3 microM) both in nonpregnant and pregnant myometria, indicating Ca2+ sensitization of contractile elements. 4. Western immunoblot analysis indicated that pregnant myometrium contained PKC isozymes such as conventional PKC (alpha, beta, gamma), novel PKC (delta, epsilon, theta), and atypical PKC (zeta but not iota and lambda). RT-PCR and real-time RT-PCR analysis indicated that, among the conventional PKC, the levels of mRNA of beta isoform in pregnant human myometrium were greater than those in nonpregnant myometrium. 5. CPI-17 is a substrate for PKC, and the phosphorylated CPI-17 is considered to inhibit myosin phosphatase. The levels of CPI-17 mRNA and protein expression were also greater in the pregnant myometrium. 6. These results suggest that the PKC-mediated contractile mechanism is augmented in human myometrium after gestation, and that this augmentation may be attributable to the increased activity of the beta PKC isoform and CPI-17.
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