ArticleThe American journal of pathology2003
Differential expression of metallothionein 1 and 2 isoforms in breast cancer lines with different invasive potential: identification of a novel nonsilent metallothionein-1H mutant variant.
Article in The American journal of pathology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 64 citations in OpenAlex.
- Analysis of lung cancer-related genetic changes in long-term and low-dose polyhexamethylene guanidine phosphate (PHMG-p) treated human pulmonary alveolar epithelial cells.BMC pharmacology & toxicology · 2022Article
- Proteomic analysis of inhibitor of apoptosis protein‑like protein‑2 on breast cancer cell proliferation.Molecular medicine reports · 2022Article
- A case-control study of Metallothionein-1 expression in breast cancer and breast fibroadenoma.Scientific reports · 2019Article
- The roles of metallothioneins in carcinogenesis.Journal of hematology & oncology · 2018Review
- Cytosolic antibody delivery by lipid-sensitive endosomolytic peptide.Nature chemistry · 2017Article
- Propiece IL-1α facilitates the growth of acute T-lymphocytic leukemia cells through the activation of NF-κB and SP1.Oncotarget · 2017Article
- Metallothionein-I/II Knockout Mice Aggravate Mitochondrial Superoxide Production and Peroxiredoxin 3 Expression in Thyroid after Excessive Iodide Exposure.Oxidative medicine and cellular longevity · 2015Article
- Genome-wide DNA methylation analysis reveals estrogen-mediated epigenetic repression of metallothionein-1 gene cluster in breast cancer.Clinical epigenetics · 2015Article
- Molecular functions of metallothionein and its role in hematological malignancies.Journal of hematology & oncology · 2012Review
- Mammalian metallothionein in toxicology, cancer, and cancer chemotherapy.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2011Review
- Time-series clustering of gene expression in irradiated and bystander fibroblasts: an application of FBPA clustering.BMC genomics · 2011Article
- Profiling of zinc-altered gene expression in human prostate normal vs. cancer cells: a time course study.The Journal of nutritional biochemistry · 2009Article
- Hypoxia-inducible factor-1alpha and vascular endothelial growth factor expression in circulating tumor cells of breast cancer patients.Breast cancer research : BCR · 2009Article
- Functional analysis of the transcription repressor PLU-1/JARID1B.Molecular and cellular biology · 2007Article
- Metallothionein isoform II expression in hyperplastic, dysplastic and neoplastic prostatic lesions.Journal of clinical pathology · 2006Article
- Quantitative immunogold study of increased expression of metallothionein-I/II in the brain perivascular areas of diabetic scrapie-infected mice.Journal of molecular histology · 2006Article
- Screening of hepatocyte proteins binding to complete S protein of hepatitis B virus by yeast-two hybrid system.World journal of gastroenterology · 2005Article
- Article
- Clinicopathological significance of metallothioneins in breast cancer.Pathology oncology research : POR · 2004Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metallothionein (MT), a low-molecular weight protein with pleiotropic functions, is believed to play an important role in tumorigenesis. The aim of this study was to compare the expression of functional MT-1 and MT-2 mRNA isoforms in five breast cancer cell lines ranging from noninvasive MCF7 breast cancer cells to highly aggressive MDA-MB-231 breast cancer cells together with breast myoepithelial cells in vitro by conventional semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and quantitative real-time RT-PCR. The MT-2A isoform was observed to be differentially upregulated in the invasive phenotype. The MT-1E isoform was found to be present in estrogen receptor-negative breast cancer cell lines (MDA-MB-231 and Hs578T) but not detectable in the estrogen receptor-positive cell lines (T47D, MCF7, and ZR75-1 cells). Only the myoepithelial cells exhibited the presence of the MT-1G transcript. Direct sequencing of the RT-PCR products revealed the occurrence of a variant MT-1H isoform with changes in amino acid residues in the protein sequence and notable differences in the predicted secondary protein structure. The observations in this study are relevant to the development of novel approaches to metastatic breast cancer disease, and may herald the search for novel MT mutants and the elucidation of their biological roles.
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