ArticleThe American journal of pathology2003
Cell-type-specific repression of the maspin gene is disrupted frequently by demethylation at the promoter region in gastric intestinal metaplasia and cancer cells.
Article in The American journal of pathology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 114 citations in OpenAlex.
- The paradoxical role of SERPINB5 in gastrointestinal cancers: oncogene or tumor suppressor?Molecular biology reports · 2025Review
- Role of class I histone deacetylases in the regulation of maspin expression in prostate cancer.Molecular carcinogenesis · 2020Article
- Homocysteine-methionine cycle is a metabolic sensor system controlling methylation-regulated pathological signaling.Redox biology · 2020Article
- An integrative study on the impact of highly differentially methylated genes on expression and cancer etiology.PloS one · 2017Article
- Epigenetic upregulation of ARL4C, due to DNA hypomethylation in the 3'-untranslated region, promotes tumorigenesis of lung squamous cell carcinoma.Oncotarget · 2016Article
- An immunohistochemical marker panel including claudin-18, maspin, and p53 improves diagnostic accuracy of bile duct neoplasms in surgical and presurgical biopsy specimens.Virchows Archiv : an international journal of pathology · 2015Article
- Epigenetics: an emerging player in gastric cancer.World journal of gastroenterology · 2014Review
- Cancer as a dysregulated epigenome allowing cellular growth advantage at the expense of the host.Nature reviews. Cancer · 2013Review
- Comparison of epigenetic profiles of human oral epithelial cells from HIV-positive (on HAART) and HIV-negative subjects.Epigenetics · 2013Article
- DNA methylation pattern as important epigenetic criterion in cancer.Genetics research international · 2013Review
- Epigenetic alterations involved in cancer stem cell reprogramming.Molecular oncology · 2012Review
- Characterization of DNA methylation and its association with other biological systems in lymphoblastoid cell lines.Genomics · 2012Article
- DNA Methylation Profiles of Protease Nexin 1 (SERPINE2) Gene in Human Cell Lines.Chinese journal of cancer research = Chung-kuo yen cheng yen chiu · 2011Article
- Clinical significance of Maspin promoter methylation and loss of its protein expression in invasive ductal breast carcinoma: correlation with VEGF-A and MTA1 expression.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2011Article
- Article
- DNA hypomethylation in the origin and pathogenesis of human diseases.Cellular and molecular life sciences : CMLS · 2009Review
- Cross talk between hedgehog and epithelial-mesenchymal transition pathways in gastric pit cells and in diffuse-type gastric cancers.British journal of cancer · 2009Article
- DNA hypomethylation arises later in prostate cancer progression than CpG island hypermethylation and contributes to metastatic tumor heterogeneity.Cancer research · 2008Article
- Invasion suppressor cystatin E/M (CST6): high-level cell type-specific expression in normal brain and epigenetic silencing in gliomas.Laboratory investigation; a journal of technical methods and pathology · 2008Article
- Aberrant epigenetic patterns in the etiology of gastrointestinal cancers.Journal of applied genetics · 2008Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Maspin, a serine protease inhibitor, was originally reported as a tumor suppressor gene in breast and prostatic cancers. We examined maspin expression and/or the allele-specific methylation status in four gastric cancer cell lines, as well as normal, metaplastic, and cancerous epithelia obtained from 50 gastric cancer patients. Three gastric cancer cell lines exhibiting maspin overexpression showed hypomethylation at either both alleles or a haploid allele. Only one cell line (GCIY) was maspin-negative but maspin expression was reactivated after treatment with a demethylating agent, 5-aza-2'-deoxycytidine. Dense and diffuse immunoreactivity for maspin was observed in 40 (80%) of 50 gastric cancers and all gastric normal epithelia (GNE) with intestinal metaplasia (IM), but not in GNE without IM. We further analyzed the allele-specific methylation status in 10 of 50 cases subjected to immunohistochemistry by the crypt isolation technique followed by a bisulfite genome sequencing method. The maspin gene promoter region of all GNE without IM was hypermethylated on both alleles whereas those with IM frequently represented the haploid type of hypomethylation status. In six of seven gastric cancers in which crypt isolation was possible, demethylation frequently occurred and extended to both alleles. Maspin mRNA was amplified from GNE with IM and cancerous crypts but not from GNE without IM. These results suggest that demethylation at the maspin gene promoter disrupts the cell-type-specific gene repression in both GNE and gastric cancer.
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