ArticleMolecular and cellular biochemistry2003
Metabolism, not autoxidation, plays a role in alpha-oxoaldehyde- and reducing sugar-induced erythrocyte GSH depletion: relevance for diabetes mellitus.
Article in Molecular and cellular biochemistry, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 53 citations in OpenAlex.
- Do We Store Packed Red Blood Cells under "Quasi-Diabetic" Conditions?Biomolecules · 2021Review
- Carbonyl Stress in Red Blood Cells and Hemoglobin.Antioxidants (Basel, Switzerland) · 2021Review
- Oxidative stress and diabetes: antioxidative strategies.Frontiers of medicine · 2020Review
- Zeaxanthin induces Nrf2-mediated phase II enzymes in protection of cell death.Cell death & disease · 2014Article
- Ethyl pyruvate prevents methyglyoxal-induced retinal vascular injury in rats.Journal of diabetes research · 2013Article
- Electrophoretic separations in poly(dimethylsiloxane) microchips using a mixture of ionic and zwitterionic surfactants.Electrophoresis · 2012Article
- 2-deoxyribose deprives cultured astrocytes of their glutathione.Neurochemical research · 2010Article
- Role of methylglyoxal in essential hypertension.The International journal of angiology : official publication of the International College of Angiology, Inc · 2010Article
- [The unsolved problem of diabetes mellitus type 2 and associated complications].Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen · 2009Review
- Personalized metabolic assessment of erythrocytes using microfluidic delivery to an array of luminescent wells.Analytical chemistry · 2009Article
- Selective detection of endogenous thiols using microchip-based flow analysis and mercury/gold amalgam microelectrodes.The Analyst · 2009Article
- A microfluidic technique for monitoring bloodstream analytes indicative of C-peptide resistance in type 2 diabetes.The Analyst · 2009Article
- Multifunctional Polypeptide EQCN Sensors: Probing the Cysteamine-Glutathione Film Permeability with Hg(II) Ions.Sensors (Basel, Switzerland) · 2008Article
- Distinct classes of glyoxalase I: metal specificity of the Yersinia pestis, Pseudomonas aeruginosa and Neisseria meningitidis enzymes.The Biochemical journal · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Erythrocyte and lens reduced glutathione (GSH) levels are often lower in patients with diabetes whereas erythrocyte dicarbonyl levels are often higher. We hypothesise that high plasma carbohydrates may be metabolised by glycolytic and pentose phosphate pathways to form alpha-oxoaldehydes, which deplete cellular GSH. Our aims were: (1) to compare the effectiveness of various carbohydrates or metabolites at depleting erythrocyte GSH, (2) to determine if GSH loss is related to the autoxidation or metabolism of carbohydrates. It was found that erythrocyte GSH was depleted by 50% (ED-50) at t = 2.5 h when erythrocytes were incubated with the following: methylglyoxal (MG) 23 microM, glyoxal 75 microM, DL-glyceraldehyde 299 microM, deoxyribose 606 microM, xylitol 626 microM, and ribose 2 mM. The glycolytic inhibitors, sodium arsenate and KF prevented ribose, deoxyribose, xylitol and MG-induced GSH depletion in erythrocytes over 2 h. However, the antioxidant trolox and the ferric chelator detapac did not affect MG-induced GSH depletion. These data suggest that the carbohydrates or glyceraldehyde were metabolised to form carbonyls such as MG which depleted erythrocyte GSH as a result of catalysis by glyoxalase I. None of the carbohydrates were autoxidised to carbonyls over this time period. We speculate that as a result of GSH depletion, subsequent glycoxidative stress affects erythrocyte function and contributes to diabetic complications.
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