ArticleNucleic acids research2003
Efficient labeling of fission yeast Schizosaccharomyces pombe with thymidine and BUdR.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 66 citations in OpenAlex.
- Transcription-replication conflict resolution by nuclear RNA interference.Molecular cell · 2025Article
- Checkpoint-Dependent Sensitivities to Nucleoside Analogues Uncover Specific Patterns of Genomic Instability.Current issues in molecular biology · 2025Article
- Telomere-to-telomere DNA replication timing profiling using single-molecule sequencing with Nanotiming.Nature communications · 2025Article
- Mrc1 regulates parental histone segregation and heterochromatin inheritance.Molecular cell · 2024Article
- Coordination of histone chaperones for parental histone segregation and epigenetic inheritance.Genes & development · 2024Article
- Multiple DNA repair pathways contribute to MMS-induced post-replicative DNA synthesis inmicroPublication biology · 2023Article
- Article
- Cosegregation of asymmetric features during cell division.Open biology · 2021Article
- Checkpoint Regulation of Nuclear Tos4 Defines S Phase Arrest in Fission Yeast.G3 (Bethesda, Md.) · 2020Article
- Telomere-binding proteins Taz1 and Rap1 regulate DSB repair and suppress gross chromosomal rearrangements in fission yeast.PLoS genetics · 2019Article
- Fission Yeast Sirtuin Hst4 Functions in Preserving Genomic Integrity by Regulating Replisome Component Mcl1.Scientific reports · 2018Article
- Global Inhibition with Specific Activation: How p53 and MYC Redistribute the Transcriptome in the DNA Double-Strand Break Response.Molecular cell · 2017Article
- Article
- RNA interactome capture in yeast.Methods (San Diego, Calif.) · 2017Article
- Sequential and counter-selectable cassettes for fission yeast.BMC biotechnology · 2016Article
- Consequences of abnormal CDK activity in S phase.Cell cycle (Georgetown, Tex.) · 2016Article
- Scarless Gene Tagging with One-Step Transformation and Two-Step Selection in Saccharomyces cerevisiae and Schizosaccharomyces pombe.PloS one · 2016Article
- Incorporation of thymidine analogs for studying replication kinetics in fission yeast.Methods in molecular biology (Clifton, N.J.) · 2015Article
- High-efficiency genome editing and allele replacement in prototrophic and wild strains of Saccharomyces.Genetics · 2014Article
- Telomere regulation during the cell cycle in fission yeast.Methods in molecular biology (Clifton, N.J.) · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In this paper we report the construction of a Schizosaccharomyces pombe strain that facilitates analysis of replicating DNA. The strain co-expresses the Herpes simplex virus thymidine kinase gene (hsv-tk) and a human equilibrative nucleoside transporter (hENT1). The double integrant efficiently incorporates 3H-thymidine into nuclear DNA as monitored by scintillation counting. These strains also incorporate the thymidine analog Bromodeoxy uridine (BUdR) into newly replicated DNA, which can be detected by immunofluorescence and flow cytometry. This strain provides a valuable tool for direct study of DNA replication in S.pombe.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.