ArticleNucleic acids research2003
Functional dissection of the mouse tyrosinase locus control region identifies a new putative boundary activity.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 44 citations in OpenAlex.
- Evolved Bmp6 enhancer alleles drive spatial shifts in gene expression during tooth development in sticklebacks.Genetics · 2021Article
- Boundary sequences flanking the mouse tyrosinase locus ensure faithful pattern of gene expression.Scientific reports · 2020Article
- Article
- Functional validation of mouse tyrosinase non-coding regulatory DNA elements by CRISPR-Cas9-mediated mutagenesis.Nucleic acids research · 2015Article
- A mobile insulator system to detect and disrupt cis-regulatory landscapes in vertebrates.Genome research · 2014Article
- Use of transgenic animals in biotechnology: prospects and problems.Acta naturae · 2013Article
- A role for insulator elements in the regulation of gene expression response to hypoxia.Nucleic acids research · 2012Article
- Regulation of the human tyrosinase gene in retinal pigment epithelium cells: the significance of transcription factor orthodenticle homeobox 2 and its polymorphic binding site.Molecular vision · 2012Article
- An insulator embedded in the chicken α-globin locus regulates chromatin domain configuration and differential gene expression.Nucleic acids research · 2011Article
- A strategy to study tyrosinase transgenes in mouse melanocytes.BMC cell biology · 2005Article
- The Tyr (albino) locus of the laboratory mouse.Mammalian genome : official journal of the International Mammalian Genome Society · 2004Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Locus control regions (LCRs) are complex high-order chromatin structures harbouring several regulatory elements, including enhancers and boundaries. We have analysed the mouse tyrosinase LCR functions, in vitro, in cell lines and, in vivo, in transgenic mice and flies. The LCR-core (2.1 kb), located at -15 kb and carrying a previously described tissue-specific DNase I hypersensitive site, operates as a transcriptional enhancer that efficiently transactivates heterologous promoters in a cell-specific orientation-independent manner. Furthermore, we have investigated the boundary activity of these sequences in transgenic animals and cells. In mice, the LCR fragment (3.7 kb) rescued a weakly expressed reference construct that displays position effects. In Drosophila, the LCR fragment and its core insulated the expression of a white minigene reporter construct from chromosomal position effects. In cells, sequences located 5' from the LCR-core displayed putative boundary activities. We have obtained genomic sequences surrounding the LCR fragment and found a LINE1 repeated element at 5'. In B16 melanoma and L929 fibroblast mouse cells, this element was found heavily methylated, supporting the existence of putative boundary elements that could prevent the spreading of condensed chromatin from the LINE1 sequences into the LCR fragment, experimentally shown to be in an open chromatin structure.
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