ArticleNucleic acids research2003
DNA mismatches and GC-rich motifs target transposition by the RAG1/RAG2 transposase.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- Insights into RAG Evolution from the Identification of "Missing Link" Family A RAGL Transposons.Molecular biology and evolution · 2023Article
- Transposable elements drive intron gain in diverse eukaryotes.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Different classes of genomic inserts contribute to human antibody diversity.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Structural basis for the activation and suppression of transposition during evolution of the RAG recombinase.The EMBO journal · 2020Article
- Functional regulation of an ancestral RAG transposon ProtoRAG by a trans-acting factor YY1 in lancelet.Nature communications · 2020Article
- How mouse RAG recombinase avoids DNA transposition.Nature structural & molecular biology · 2020Article
- Article
- New insights into the evolutionary origins of the recombination-activating gene proteins and V(D)J recombination.The FEBS journal · 2017Review
- RAG1/2 induces genomic insertions by mobilizing DNA into RAG1/2-independent breaks.The Journal of experimental medicine · 2017Article
- Article
- Target capture during Mos1 transposition.The Journal of biological chemistry · 2014Article
- V(D)J recombination: Born to be wild.Seminars in cancer biology · 2010Review
- Sequence features of HLA-DRB1 locus define putative basis for gene conversion and point mutations.BMC genomics · 2008Article
- Understanding how the V(D)J recombinase catalyzes transesterification: distinctions between DNA cleavage and transposition.Nucleic acids research · 2008Article
- The structure-specific nicking of small heteroduplexes by the RAG complex: implications for lymphoid chromosomal translocations.DNA repair · 2007Article
- Target DNA structure plays a critical role in RAG transposition.PLoS biology · 2006Article
- Genomic instability due to V(D)J recombination-associated transposition.Genes & development · 2006Article
- Mobilization of RAG-generated signal ends by transposition and insertion in vivo.Molecular and cellular biology · 2006Article
- Double-strand break formation by the RAG complex at the bcl-2 major breakpoint region and at other non-B DNA structures in vitro.Molecular and cellular biology · 2005Article
- True reversal of Mu integration.The EMBO journal · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In addition to their essential role in V(D)J recombination, the RAG proteins function as a transposase capable of inserting the V(D)J recombination intermediate, the signal end DNA fragment, into target DNA. RAG-mediated transposition has been suggested to contribute to genome instability and the development of lymphoid malignancies. Previous studies suggested that the RAG transposase exhibits a target site preference for GC rich sequences and hairpin structures. Here we demonstrate that a transposition hot spot (5'-GCCGCCGGGCC-3'), smaller portions of this hot spot and other GC rich motifs are able to target RAG-mediated transposition. Tracks of GC base pairs have been shown to have an unusually high rate of base pair breathing. Intriguingly, we find that DNA mismatches can efficiently target RAG-mediated transposition and suppress the use of other target sites. Hairpins, however, are not generally preferred targets. Our results indicate that target DNA melting may be a crucial step during RAG-mediated transposition, and that target site selection by the RAG transposase may be intimately linked to mutagenic and metabolic processes that transiently present favorable DNA structures to the transposition machinery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.