ArticleNucleic acids research2003
Identification of peptide inhibitors of pre-mRNA splicing derived from the essential interaction domains of CDC5L and PLRG1.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Prp19/CDC5L promotes gastric cancer via activation of the MAPK pathway-mediated homologous recombination.International journal of biological sciences · 2025Article
- Article
- Biology of the mRNA Splicing Machinery and Its Dysregulation in Cancer Providing Therapeutic Opportunities.International journal of molecular sciences · 2021Review
- Gene regulation could be attributed to TCF3 and other key transcription factors in the muscle of pubertal heifers.Veterinary medicine and science · 2020Article
- Prp19 Arrests Cell Cycle via Cdc5L in Hepatocellular Carcinoma Cells.International journal of molecular sciences · 2017Article
- Expression and Clinical Role of Cdc5L as a Novel Cell Cycle Protein in Hepatocellular Carcinoma.Digestive diseases and sciences · 2016Article
- Expression of CDC5L is associated with tumor progression in gliomas.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Small Molecule Modulators of Pre-mRNA Splicing in Cancer Therapy.Trends in molecular medicine · 2016Review
- Depletion of pre-mRNA splicing factor Cdc5L inhibits mitotic progression and triggers mitotic catastrophe.Cell death & disease · 2014Article
- Blom7alpha is a novel heterogeneous nuclear ribonucleoprotein K homology domain protein involved in pre-mRNA splicing that interacts with SNEVPrp19-Pso4.The Journal of biological chemistry · 2009Article
- Two Prp19-like U-box proteins in the MOS4-associated complex play redundant roles in plant innate immunity.PLoS pathogens · 2009Article
- PLRG1 is an essential regulator of cell proliferation and apoptosis during vertebrate development and tissue homeostasis.Molecular and cellular biology · 2009Article
- SNEV is an evolutionarily conserved splicing factor whose oligomerization is necessary for spliceosome assembly.Nucleic acids research · 2005Article
Corrections and comments
- Commented on by
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
CDC5L and PLRG1 are both spliceosomal proteins that are highly conserved across species. They have both been shown to be part of sub- spliceosomal protein complexes that are essential for pre-mRNA splicing in yeast and humans. CDC5L and PLRG1 interact directly in vitro. This interaction is mediated by WD40 regions in PLRG1 and the C-terminal domain of CDC5L. In order to determine whether this interaction is important for the splicing mechanism, we have designed peptides corresponding to highly conserved sequences in the interaction domains of both proteins. These peptides were used in in vitro splicing experiments as competitors to the cognate sequences in the endogenous proteins. Certain peptides derived from the binding domains of both proteins were found to inhibit in vitro splicing. This splicing inhibition could be prevented by preincubating the peptides with the corresponding partner protein that had been expressed in Escherichia coli. The results from this study indicate that the interaction between CDC5L and PLRG1 is essential for pre-mRNA splicing and further demonstrate that small peptides can be used as effective splicing inhibitors.
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