Evidence map›Paper›PMID 14576130›Full record

ArticleAntimicrobial agents and chemotherapy2003

Rapid detection and estimation by pyrosequencing of 23S rRNA genes with a single nucleotide polymorphism conferring linezolid resistance in Enterococci.

Alistair Sinclair, Catherine Arnold, Neil Woodford

Open access · greenAbstract read
In one paragraph

Article in Antimicrobial agents and chemotherapy, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 97 citations in OpenAlex.

  1. Linezolid in the Focus of Antimicrobial Resistance ofInternational journal of molecular sciences · 2025
    Review
  2. Article
  3. Review
  4. Vancomycin Resistance inMicroorganisms · 2022
    Review
  5. Article
  6. Article
  7. Genome-Based Prediction of Bacterial Antibiotic Resistance.Journal of clinical microbiology · 2019
    Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Molecular analysis of linezolid resistance in clinical Enterococcus faecium isolates by polymerase chain reaction and pyrosequencing.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011
    Article
  14. Article
  15. Article
  16. Pyrosequencing assay to rapidly detect clarithromycin resistance mutations in Canadian Helicobacter pylori isolates.Canadian journal of gastroenterology = Journal canadien de gastroenterologie · 2009
    Article
  17. Article
  18. Endemic linezolid-resistant Staphylococcus epidermidis in a critical care unit.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2009
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Alistair SinclairAntibiotic Resistance Monitoring and Reference Laboratory, Specialist and Reference Microbiology Division, Health Protection Agency-Colindale, London NW9 5HT, United Kingdom.
Catherine Arnold
Neil Woodford
Genomics (United Kingdom) · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pyrosequencing was used to detect rapidly and estimate the number of 23S rRNA genes with a G2576T mutation in 43 linezolid-resistant and -susceptible clinical isolates of enterococci. The method showed 100% concordance with PCR-restriction fragment length polymorphism for detecting isolates homozygous for either G2576 or T2576 or heterozygous for this mutation. A good correlation was found between linezolid MICs and the number of 23S rRNA gene copies carrying the mutation.

Indexed as

AcetamidesAnti-Infective AgentsDrug Resistance, BacterialEnterococcusGenes, BacterialLinezolidMutationOxazolidinonesPolymorphism, Single NucleotideReverse Transcriptase Polymerase Chain ReactionRNA, Ribosomal, 23SAcetamidesAnti-Infective AgentsLinezolidOxazolidinonesRNA, Ribosomal, 23S

Identifiers

PMID14576130
PMCPMC253796
OpenAlexW2159864819

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.