ArticleEuropean biophysics journal : EBJ2004
Fluorescence spectroscopic detection of mitochondrial flavoprotein redox oscillations and transient reduction of the NADPH oxidase-associated flavoprotein in leukocytes.
Article in European biophysics journal : EBJ, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Quantitative Flavoprotein Fluorescence Parameters in Retinal and Optic Nerve Diseases: A Scoping Review.Journal of clinical medicine · 2026Review
- Flavoprotein Fluorescence Imaging in Stargardt Disease: Linking Metabolic Stress to Structural Damage.Investigative ophthalmology & visual science · 2025Article
- Watching synchronous mitochondrial respiration in the retina and its instability in a mouse model of macular degeneration.Scientific reports · 2021Article
- Discovery and clinical translation of novel glaucoma biomarkers.Progress in retinal and eye research · 2021Review
- Neutrophil fluorescence in clozapine users is attributable to a 14kDa secretable protein.Pharmacology research & perspectives · 2020Article
- Spectrally and spatially resolved laser-induced photobleaching of endogenous flavin fluorescence in cardiac myocytes.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- Imaging Retinal Ganglion Cell Death and Dysfunction in Glaucoma.International ophthalmology clinics · 2019Review
- Hyperspectral fluorescence microscopy detects autofluorescent factors that can be exploited as a diagnostic method for Candida species differentiation.Journal of biomedical optics · 2017Article
- Optical imaging of mitochondrial redox state in rodent model of retinitis pigmentosa.Journal of biomedical optics · 2013Article
- The bisretinoids of retinal pigment epithelium.Progress in retinal and eye research · 2012Review
- PROBING THE IMPACT OF GAMMA-IRRADIATION ON THE METABOLIC STATE OF NEURAL STEM AND PRECURSOR CELLS USING DUAL-WAVELENGTH INTRINSIC SIGNAL TWO-PHOTON EXCITED FLUORESCENCE.Journal of innovative optical health sciences · 2011Article
- Topographic and age-related changes of the retinal epithelium and Bruch's membrane of rhesus monkeys.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2010Article
- Detection of retinal metabolic stress resulting from central serous retinopathy.Retina (Philadelphia, Pa.) · 2009Article
- Metal-enhanced chemiluminescence: advanced chemiluminescence concepts for the 21st century.Chemical Society reviews · 2009Review
- Rapid, noninvasive detection of diabetes-induced retinal metabolic stress.Archives of ophthalmology (Chicago, Ill. : 1960) · 2008Article
- Retinal flavoprotein autofluorescence as a measure of retinal health.Transactions of the American Ophthalmological Society · 2008Article
- Elevated glucose concentrations promote receptor-independent activation of adherent human neutrophils: an experimental and computational approach.Biophysical journal · 2007Article
- Regionalization of plasma membrane-bound flavoproteins of cerebellar granule neurons in culture by fluorescence energy transfer imaging.Journal of fluorescence · 2006Article
- Real-time control of neutrophil metabolism by very weak ultra-low frequency pulsed magnetic fields.Biophysical journal · 2005Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Steady-state and time-resolved fluorescence spectroscopy and fluorescence microscopy of leukocyte flavoproteins have been performed. Both living human peripheral blood monocytes and neutrophils have been utilized as experimental models, as the former relies much more heavily on mitochondrial metabolism for energy production than the latter. We confirm previous studies indicating that cellular flavoproteins absorb at 460 nm and emit at 530 nm, very similar to that of the FAD moiety. Furthermore, the emission properties of intracellular flavoproteins were altered by the metabolic inhibitors rotenone, antimycin A, azide, cyanide, DNP (2,4-dinitrophenol), and FCCP [carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone]. Kinetic studies revealed flavoprotein emission oscillations in both monocytes and neutrophils. The flavoprotein intensity oscillations correlated with the physiological status of the cell and the nature of membrane receptor ligation. Microscopy revealed the presence of flavoprotein fluorescence in association with the plasma membrane, intracellular granules and distributed throughout the cytoplasm, presumably within mitochondria. Metabolic inhibitors such as cyanide suggest that the plasma membrane and granular components are cyanide-insensitive and therefore are likely associated with the flavoprotein component of the NADPH oxidase, which is located in these two compartments. This interpretation was found to be consistent with structural localization of the NADPH oxidase using an antibody molecule specific for this protein. Using peripheral blood neutrophils, which display less active mitochondria, and time-resolved emission spectroscopy, we show that the NADPH oxidase-associated flavoprotein undergoes a periodic transient reduction of about 54+/-2 ms in living cells. This finding is consistent with prior studies indicating that propagating substrate (NADPH) waves periodically promote electron transport across the NADPH oxidase.
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