ArticleInfection and immunity2003
Lung epithelial cells and extracellular matrix components induce expression of Pneumocystis carinii STE20, a gene complementing the mating and pseudohyphal growth defects of STE20 mutant yeast.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Exploring microorganism-host interactions: Emerging organoid models and analytical approaches.mLife · 2026Review
- Lung Epithelial Cell Line Immune Responses toJournal of fungi (Basel, Switzerland) · 2023Review
- Gene expression in lung epithelial cells following interaction with Pneumocystis carinii and its specific life forms yields insights into host gene responses to infection.Microbiology and immunology · 2022Article
- Recent Advances in Fungal Infections: From Lung Ecology to Therapeutic Strategies With a Focus onFrontiers in medicine · 2022Review
- Acyl-CoA-binding protein (ACBP): a phylogenetically conserved appetite stimulator.Cell death & disease · 2020Article
- An Overview of the Function and Maintenance of Sexual Reproduction in Dikaryotic Fungi.Frontiers in microbiology · 2018Review
- Regulatory roles of phosphorylation in model and pathogenic fungi.Medical mycology · 2016Review
- Evidence for a Pneumocystis carinii Flo8-like transcription factor: insights into organism adhesion.Medical microbiology and immunology · 2016Article
- Pneumocystis.Cold Spring Harbor perspectives in medicine · 2014Review
- The Pneumocystis Ace2 transcription factor regulates cell wall-remodeling genes and organism virulence.The Journal of biological chemistry · 2013Article
- Substrate analysis of the Pneumocystis carinii protein kinases PcCbk1 and PcSte20 using yeast proteome microarrays provides a novel method for Pneumocystis signalling biology.Yeast (Chichester, England) · 2011Article
- Pneumocystis carinii interactions with lung epithelial cells and matrix proteins induce expression and activity of the PcSte20 kinase with subsequent phosphorylation of the downstream cell wall biosynthesis kinase PcCbk1.Infection and immunity · 2011Article
- Characterization of PCEng2, a {beta}-1,3-endoglucanase homolog in Pneumocystis carinii with activity in cell wall regulation.American journal of respiratory cell and molecular biology · 2010Article
- Characterization of the PcCdc42 small G protein from Pneumocystis carinii, which interacts with the PcSte20 life cycle regulatory kinase.American journal of physiology. Lung cellular and molecular physiology · 2010Article
- The Pneumocystis meiotic PCRan1p kinase exhibits unique temperature-regulated activity.American journal of respiratory cell and molecular biology · 2009Article
- Characterization of a novel ADAM protease expressed by Pneumocystis carinii.Infection and immunity · 2009Article
- Pneumocystis workshop: 10th anniversary summary.Eukaryotic cell · 2009Article
- Parenchymal cell TNF receptors contribute to inflammatory cell recruitment and respiratory failure in Pneumocystis carinii-induced pneumonia.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- Pneumocystis carinii exhibits a conserved meiotic control pathway.Infection and immunity · 2008Article
- Purification and partial characterization of a Paracoccidioides brasiliensis protein with capacity to bind to extracellular matrix proteins.Infection and immunity · 2005Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Pneumocystis carinii causes severe pneumonia in immunocompromised hosts. The binding of P. carinii to alveolar epithelial cells and extracellular matrix constituents such as fibronectin and vitronectin is a central feature of infection, which initiates proliferation of the organism. Herein, we demonstrate that P. carinii binding to lung cells specifically alters the gene expression of the organism, regulating fungal growth. Subtractive hybridization was performed to isolate P. carinii genes expressed following binding to mammalian extracellular matrix constituents. P. carinii STE20 (PCSTE20), a gene participating in mating and pseudohyphal growth of other fungi, was identified following adherence to the extracellular matrix constituents fibronectin, vitronectin, collagen, and lung epithelial cells. The expression of PCSTE20 and a related P. carinii mitogen-activated protein kinase (MAPK) kinase gene, also implicated in signaling of mating, were both specifically upregulated by binding to matrix protein. The expression of general cyclin-dependent kinases and other MAPKs not involved in mating pathways were not altered by organism binding. PCSTE20 expression was also strongly enhanced following organism attachment to A549 lung epithelial cells. When expressed in a Saccharomyces cerevisiae ste20Delta mutant, PCSTE20 suppressed defects in both mating and pseudohyphal growth. These findings are consistent with the observed proliferation and filopodial extension of Pneumocystis organisms adherent to the epithelium in the lungs of immunocompromised hosts. PCSTE20 expression appears to represent a significant component in the regulation of the life cycle of this intractable opportunistic pathogen.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.